Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes

Recruitment of galectin-3 during cell invasion and intracellular trafficking of Trypanosoma cruzi extracellular amastigotes

Author Machado, Fabricio Castro Google Scholar
Cruz, Lilian Google Scholar
Silva, Aline Alves da Google Scholar
Cruz, Mario Costa Autor UNIFESP Google Scholar
Mortara, Renato Arruda Autor UNIFESP Google Scholar
Roque-Barreira, Maria Cristina Google Scholar
Silva, Claudio Vieira da Google Scholar
Institution Universidade Federal de Uberlândia (UFU)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The invasion of host cells by the intracellular protozoan Trypanosoma cruzi requires interactions with host cell molecules, and the replication of the parasite requires escape from a parasitophorous vacuole into the host cell cytosol. Galectin-3, a member of beta-galactosidase-binding lectin family, has numerous extracellular and intracellular functions. in this study, we investigated the role of galectin-3 during the invasion and intracellular trafficking of T. cruzi extracellular amastigotes (EAs). Endogenous galectin-3 from mouse peritoneal macrophages accumulated around the pathogen during cell invasion by EAs. in addition, galectin-3 accumulated around parasites after their escape from the parasitophorous vacuole. Thus, galectin-3 behaved as a novel marker of phagolysosome lysis during the infection of host cells by T. cruzi.
Keywords galectin-3
intracellular traffic
phagolysosome lysis
Trypanosoma cruzi
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPEMIG: PIBIC B 004/2009
FAPEMIG: APQ-00621-11
FAPEMIG: APQ-00305-12
CAPES: 23038.005295/2011-40
Date 2014-02-01
Published in Glycobiology. Cary: Oxford Univ Press Inc, v. 24, n. 2, p. 179-184, 2014.
ISSN 0959-6658 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press Inc
Extent 179-184
Origin http://dx.doi.org/10.1093/glycob/cwt097
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000330839300007
URI http://repositorio.unifesp.br/handle/11600/37340

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