Ovariectomy and 17 beta-estradiol replacement play a role on the expression of Endonuclease-G and phosphorylated cyclic AMP response element-binding (CREB) protein in hippocampus

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dc.contributor.author Santos Pereira, Renato Tavares dos
dc.contributor.author Porto, Catarina Segreti [UNIFESP]
dc.contributor.author Francis Abdalla, Fernando Mauricio
dc.date.accessioned 2016-01-24T14:35:10Z
dc.date.available 2016-01-24T14:35:10Z
dc.date.issued 2014-01-25
dc.identifier http://dx.doi.org/10.1016/j.mce.2013.09.037
dc.identifier.citation Molecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 382, n. 1, p. 227-233, 2014.
dc.identifier.issn 0303-7207
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37329
dc.description.abstract The aim of the present study was to investigate the effects of different periods of ovariectomy and 17 beta-estradiol (E2) replacement on the expression of Cytochrome C, apoptosis inducing factor (AIF) and Endonuclease-G (Endo-G) in mitochondrial and cytosolic fractions obtained from hippocampus of the adult female rats. in addition, the expression of phosphorylated CREB (phospho-CREB) was also analyzed in hippocampus. Ovariectomy or E2 treatment did not change the expression of Cytochrome C and AIF. Ovariectomy (15, 21 and 36 days) decreased the expression of Endo-G in the mitochondrial fractions and increased it in the cytosolic fractions obtained from hippocampus. the treatment with E2 after 15 days of ovariectomy for 7 days or 21 days, and throughout the post-ovariectomy period prevented the effects of ovariectomy on Endo-G expression. Our results suggest that ovariectomy-induced apoptotic cell death in hippocampal tissue could be mediated by Endo-G, but not by AIF, via a caspase-independent apoptotic pathway. Furthermore, ovariectomy decreased the expression of phospho-CREB and the treatment with E2 prevented these effects. in conclusion, E2 may help maintain long-term neuronal viability by regulating the expression of members of the Bcl-2 family. Regulation of Endo-G released from mitochondria, but not of Cytochrome C and AIF, is also involved in the neuroprotective actions of E2. Furthermore, CREB may be involved in the expression of Bcl-2. These data provide new understanding into the mechanisms involved in the neuroprotective role of estrogen. (C) 2013 Elsevier Ireland Ltd. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 227-233
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Molecular and Cellular Endocrinology
dc.rights Acesso restrito
dc.subject Endonuclease-G en
dc.subject Cytochrome C en
dc.subject AIF en
dc.subject CREB en
dc.subject Hippocampus en
dc.subject 17 beta-Estradiol en
dc.title Ovariectomy and 17 beta-estradiol replacement play a role on the expression of Endonuclease-G and phosphorylated cyclic AMP response element-binding (CREB) protein in hippocampus en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Inst Butantan
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Inst Butantan, Pharmacol Lab, BR-05503900 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Pharmacol, Sect Expt Endocrinol, São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 08/56564-1
dc.identifier.doi 10.1016/j.mce.2013.09.037
dc.description.source Web of Science
dc.identifier.wos WOS:000330421600024



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