Ovariectomy and 17 beta-estradiol replacement play a role on the expression of Endonuclease-G and phosphorylated cyclic AMP response element-binding (CREB) protein in hippocampus

Ovariectomy and 17 beta-estradiol replacement play a role on the expression of Endonuclease-G and phosphorylated cyclic AMP response element-binding (CREB) protein in hippocampus

Author Santos Pereira, Renato Tavares dos Google Scholar
Porto, Catarina Segreti Autor UNIFESP Google Scholar
Francis Abdalla, Fernando Mauricio Google Scholar
Institution Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of the present study was to investigate the effects of different periods of ovariectomy and 17 beta-estradiol (E2) replacement on the expression of Cytochrome C, apoptosis inducing factor (AIF) and Endonuclease-G (Endo-G) in mitochondrial and cytosolic fractions obtained from hippocampus of the adult female rats. in addition, the expression of phosphorylated CREB (phospho-CREB) was also analyzed in hippocampus. Ovariectomy or E2 treatment did not change the expression of Cytochrome C and AIF. Ovariectomy (15, 21 and 36 days) decreased the expression of Endo-G in the mitochondrial fractions and increased it in the cytosolic fractions obtained from hippocampus. the treatment with E2 after 15 days of ovariectomy for 7 days or 21 days, and throughout the post-ovariectomy period prevented the effects of ovariectomy on Endo-G expression. Our results suggest that ovariectomy-induced apoptotic cell death in hippocampal tissue could be mediated by Endo-G, but not by AIF, via a caspase-independent apoptotic pathway. Furthermore, ovariectomy decreased the expression of phospho-CREB and the treatment with E2 prevented these effects. in conclusion, E2 may help maintain long-term neuronal viability by regulating the expression of members of the Bcl-2 family. Regulation of Endo-G released from mitochondria, but not of Cytochrome C and AIF, is also involved in the neuroprotective actions of E2. Furthermore, CREB may be involved in the expression of Bcl-2. These data provide new understanding into the mechanisms involved in the neuroprotective role of estrogen. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
Keywords Endonuclease-G
Cytochrome C
AIF
CREB
Hippocampus
17 beta-Estradiol
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 08/56564-1
Date 2014-01-25
Published in Molecular and Cellular Endocrinology. Clare: Elsevier B.V., v. 382, n. 1, p. 227-233, 2014.
ISSN 0303-7207 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 227-233
Origin http://dx.doi.org/10.1016/j.mce.2013.09.037
Access rights Closed access
Type Article
Web of Science ID WOS:000330421600024
URI http://repositorio.unifesp.br/handle/11600/37329

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