Gene Expression in B-1 Cells from Lupus-Prone Mice

Gene Expression in B-1 Cells from Lupus-Prone Mice

Author Novaes e Brito, Ronni Romulo Autor UNIFESP Google Scholar
Xander, Patricia Autor UNIFESP Google Scholar
Perez, Elizabeth C. Autor UNIFESP Google Scholar
Maricato, Juliana Terzi Autor UNIFESP Google Scholar
Laurindo, Maria Fl. Autor UNIFESP Google Scholar
De Lorenzo, Beatriz H. P. Autor UNIFESP Google Scholar
Pellegrino, Renata Autor UNIFESP Google Scholar
Bernardo, Viviane Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Ctr Univ Sao Camilo
Abstract New Zealand Black X New Zealand White F1 [(NZB/NZW) F1] mice develop an autoimmune condition with similarities to human systemic lupus erythematosus (SLE). in this study, we demonstrate that B-1 cells, which have previously been reported to be involved in several autoimmune diseases, have altered gene expression in these mice. RNA was extracted from purified B-1 cells of disease-free C57BL/6 mice and lupus-prone (NZB/NZW) F1 mice. Gene expression was analysed using DNA microarray techniques and validated by real time reverse transcriptase polymerase chain reaction (RT-PCR). in (NZB/NZW) F1 mice, some genes had altered expression patterns compared to disease-free controls. Specifically, the upregulation of Ifitm1, Pvrl2 and Ifi202b and downregulation of Trp53bp1 mRNA were observed in (NZB/NZW) F1 mice. These genes are known to be associated with autoimmune diseases. This pattern of gene expression in B-1 cells could understanding of the pathogenesis of SLE. Thus, it is reasonable to hypothesise that the altered gene expression observed in B-1 cells in our experimental model is important for SLE prognosis and therapy, and these implications are discussed herein.
Keywords Autoimmunity
B-1 cells
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2014-01-01
Published in Immunological Investigations. London: Informa Healthcare, v. 43, n. 7, p. 675-692, 2014.
ISSN 0882-0139 (Sherpa/Romeo, impact factor)
Publisher Informa Healthcare
Extent 675-692
Access rights Closed access
Type Article
Web of Science ID WOS:000341303600006

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