The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion

The effects of 780-nm low-level laser therapy on muscle healing process after cryolesion

Author Brunelli, Roberta M. Google Scholar
Rodrigues, Natalia C. Google Scholar
Ribeiro, Daniel A. Autor UNIFESP Google Scholar
Fernandes, Kelly Autor UNIFESP Google Scholar
Magri, Angela Autor UNIFESP Google Scholar
Assis, Livia Autor UNIFESP Google Scholar
Parizotto, Nivaldo A. Google Scholar
Cliquet, Alberto Google Scholar
Renno, Ana Claudia M. Autor UNIFESP Google Scholar
Abreu, Daniela C. C. Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Carlos (UFSCar)
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The objective of this study was to assess the effects of 780-nm low-level laser therapy at different periods of 7, 14 and 21 days after cryolesion, including the dose (10 or 50 J/cm(2)), to promote a better muscle repair evidenced by histopathological and immunohistochemical analyses. Fifty-four male rats were divided into three groups: injured control group (CG)-injured animals without any treatment; injured 780-nm laser-treated group, at 10 J/cm(2) (G10); and injured 780-nm laser-treated group, at 50 J/cm(2) (G50). Each group was divided into three subgroups (n = 6): 7, 14 and 21 days post-injury. Histopathological findings revealed better organised muscle fibres in the G10 and G50 during the periods of 7 and 14 days compared to the CG. the G10 and G50 during the 7 days showed a significant reduction (p < 0.05) of lesion area compared to the CG, without differences between groups treated for 14 and 21 days. the G10 showed an increase of the amount of vessels after 14 days compared to the G50, but not in relation to controls. With regard to the immunohistochemical analyses of the MyoD factor, the G10 and G50 during the 7 days showed higher concentrations of immunomarkers than controls. Myogenin immunomarkers were similarly observed at days 7 and 14 in all the three groups analysed, whereas immunomarkers were found in none of the groups after 21 days of laser therapy. the results showed that laser, regardless the applied dose, has positive effects on muscle repair.
Keywords Physical resource
Qualitative histological analysis
Vessel counting
Lesion area
MyoD
Myogenin
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2014-01-01
Published in Lasers in Medical Science. London: Springer London Ltd, v. 29, n. 1, p. 91-96, 2014.
ISSN 0268-8921 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 91-96
Origin http://dx.doi.org/10.1007/s10103-013-1277-6
Access rights Closed access
Type Article
Web of Science ID WOS:000329834200012
URI http://repositorio.unifesp.br/handle/11600/37227

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