Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain in Vitro and in Vivo and the Influence of Type Three Secretion System

Analysis of the Virulence of an Atypical Enteropathogenic Escherichia coli Strain in Vitro and in Vivo and the Influence of Type Three Secretion System

Author Sampaio, Suely C. F. Autor UNIFESP Google Scholar
Moreira, Fabiana C. Autor UNIFESP Google Scholar
Liberatore, Ana M. A. Autor UNIFESP Google Scholar
Vieira, Monica A. M. Autor UNIFESP Google Scholar
Knobl, Terezinha Google Scholar
Romao, Fabiano T. Autor UNIFESP Google Scholar
Hernandes, Rodrigo T. Autor UNIFESP Google Scholar
Ferreira, Claudete S. A. Google Scholar
Ferreira, Antonio P. Google Scholar
Felipe-Silva, Aloisio Google Scholar
Sinigaglia-Coimbra, Rita Autor UNIFESP Google Scholar
Koh, Ivan H. J. Autor UNIFESP Google Scholar
Gomes, Tania A. T. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Univ Estadual Paulista
Fleury Med & Saude
Abstract Atypical enteropathogenic Escherichia coli (aEPEC) inject various effectors into intestinal cells through a type three secretion system (T3SS), causing attaching and effacing (A/E) lesions. We investigated the role of T3SS in the ability of the aEPEC 1711-4 strain to interact with enterocytes in vitro (Caco-2 cells) and in vivo (rabbit ileal loops) and to translocate the rat intestinal mucosa in vivo. A T3SS isogenic mutant strain was constructed, which showed marked reduction in the ability to associate and invade but not to persist inside Caco-2 cells. After rabbit infection, only aEPEC 1711-4 was detected inside enterocytes at 8 and 24 hours pointing to a T3SS-dependent invasive potential in vivo. in contrast to aEPEC 1711-4, the T3SS-deficient strain no longer produced A/E lesions or induced macrophage infiltration. We also demonstrated that the ability of aEPEC 1711-4 to translocate through mesenteric lymph nodes to spleen and liver in a rat model depends on a functional T3SS, since a decreased number of T3SS mutant bacteria were recovered from extraintestinal sites. These findings indicate that the full virulence potential of aEPEC 1711-4 depends on a functional T3SS, which contributes to efficient adhesion/invasion in vitro and in vivo and to bacterial translocation to extraintestinal sites.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2008/53812-4
FAPESP: 2011/12664-5
CNPq: 304453/2011-0
CNPq: 150833/2012-1
Date 2014-01-01
Published in Biomed Research International. New York: Hindawi Publishing Corporation, 9 p., 2014.
ISSN 2314-6133 (Sherpa/Romeo, impact factor)
Publisher Hindawi Publishing Corporation
Extent 9
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000336293900001

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