TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA

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dc.contributor.author Fernandes, Elizabeth S.
dc.contributor.author Brito, Carolina X. L.
dc.contributor.author Teixeira, Simone A.
dc.contributor.author Barboza, Renato [UNIFESP]
dc.contributor.author Reis, Aramys S. dos
dc.contributor.author Azevedo-Santos, Ana Paula S.
dc.contributor.author Muscara, Marcelo
dc.contributor.author Costa, Soraia K. P.
dc.contributor.author Marinho, Claudio R. F.
dc.contributor.author Brain, Susan D.
dc.contributor.author Grisotto, Andmarcos A. G.
dc.date.accessioned 2016-01-24T14:34:56Z
dc.date.available 2016-01-24T14:34:56Z
dc.date.issued 2014-01-01
dc.identifier http://dx.doi.org/10.1155/2014/506450
dc.identifier.citation Mediators of Inflammation. New York: Hindawi Publishing Corporation, 12 p., 2014.
dc.identifier.issn 0962-9351
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37141
dc.description.abstract Thousands of people suffer from severe malaria every year. the innate immune response plays a determinant role in host's defence to malaria. Transient receptor potential vanilloid 1 (TRPV1) modulates macrophage-mediated responses in sepsis, but its role in other pathogenic diseases has never been addressed. We investigated the effects of capsazepine, a TRPV1 antagonist, in malaria. C57BL/6 mice received 10(5) red blood cells infected with Plasmodium berghei ANKA intraperitoneally. Noninfected mice were used as controls. Capsazepine or vehicle was given intraperitoneally for 6 days. Mice were culled on day 7 after infection and blood and spleen cell phenotype and activation were evaluated. Capsazepine decreased circulating but not spleen F4/80(+)Ly6G(+) cell numbers as well as activation of both F4/80(+) and F4/80(+)Ly6G(+) cells in infected animals. in addition, capsazepine increased circulating but not spleen GR1(+) and natural killer (NK) population, without interfering with natural killer T (NKT) cell numbers and blood NK and NKT activation. However, capsazepine diminished CD69 expression in spleen NKT but not NK cells. Infection increased lipid peroxidation and the release of TNF alpha and IFN gamma, although capsazepine-treated group exhibited lower levels of lipid peroxidation and TNF alpha. Capsazepine treatment did not affect parasitaemia. Overall, TRPV1 antagonism modulates the innate immune response to malaria. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundacao de Amparo a Pesquisa e Desenvolvimento Cientifico do Maranhao (FAPEMA; Brazil)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 12
dc.language.iso eng
dc.publisher Hindawi Publishing Corporation
dc.relation.ispartof Mediators of Inflammation
dc.rights Acesso aberto
dc.title TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA en
dc.type Artigo
dc.contributor.institution Univ CEUMA
dc.contributor.institution Kings Coll London
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Fed Maranhao
dc.contributor.institution Inst Florence Ensino Super
dc.description.affiliation Univ CEUMA, BR-65075120 Sao Luis, MA, Brazil
dc.description.affiliation Kings Coll London, Div Cardiovasc, London WC2R 2LS, England
dc.description.affiliation Univ São Paulo, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Diadema, Brazil
dc.description.affiliation Univ Fed Maranhao, Sao Luis, Brazil
dc.description.affiliation Inst Florence Ensino Super, Sao Luis, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Diadema, Brazil
dc.identifier.file WOS000342942500001.pdf
dc.identifier.doi 10.1155/2014/506450
dc.description.source Web of Science
dc.identifier.wos WOS:000342942500001



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