Effects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis

Effects of phototherapy on cartilage structure and inflammatory markers in an experimental model of osteoarthritis

Author Oliveira, Poliani Google Scholar
Santos, Anderson Amaro Google Scholar
Rodrigues, Tamara Google Scholar
Tim, Carla Roberta Google Scholar
Pinto, Karina Zambone Autor UNIFESP Google Scholar
Paiva Magri, Angela Maria Autor UNIFESP Google Scholar
Fernandes, Kelly Rossetti Autor UNIFESP Google Scholar
Mattiello, Stela M. Google Scholar
Parizotto, Nivaldo Antonio Google Scholar
Anibal, Fernanda Freitas Autor UNIFESP Google Scholar
Muniz Renno, Ana Claudia Autor UNIFESP Google Scholar
Institution Universidade Federal de São Carlos (UFSCar)
Adventist Univ Ctr São Paulo
Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to evaluate the effects of laser phototherapy on the degenerative modifications on the articular cartilage after the anterior cruciate ligament transection (ACLT) in the knee of rats. Eighty male rats (Wistar) were distributed into four groups: intact control group (IG), injured control group (CG), injured laser treated group at 10 J/cm(2) (L10), and injured laser treated group at 50 J/cm(2) (L50). Animals were distributed into two subgroups, sacrificed in 5 and 8 weeks postsurgery. the ACLT was used to induce knee osteoarthritis in rats. After 2 weeks postsurgery, laser phototherapy initiated and it was performed for 15 and 30 sessions. the histological findings revealed that laser irradiation, especially at 10 J/cm(2), modulated the progression of the degenerative process, showing a better cartilage structure and lower number of condrocytes compared to the other groups. Laser phototherapy was not able to decrease the degenerative process measured by Mankin score and prevent the increase of cartilage thickness related to the degenerative process. Moreover, it did not have any effect in the biomodulation of the expression of markers IL1 beta, tumor necrosis factor-alpha, and metalloprotein-13. Furthermore, laser irradiated animals, at 50 J/cm(2) showed a lower amount of collagen type 1. (C) 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Keywords articular cartilage
chondrocytes
laser irradiation
osteoarthritis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-12-01
Published in Journal of Biomedical Optics. Bellingham: Spie-soc Photo-optical Instrumentation Engineers, v. 18, n. 12, 10 p., 2013.
ISSN 1083-3668 (Sherpa/Romeo, impact factor)
Publisher Spie-soc Photo-optical Instrumentation Engineers
Extent 10
Origin http://dx.doi.org/10.1117/1.JBO.18.12.128004
Access rights Closed access
Type Article
Web of Science ID WOS:000331706500051
URI http://repositorio.unifesp.br/handle/11600/37064

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