Morphometric evaluation of the fetal rat liver after maternal dexamethasone treatment: effect on the maturation of erythroid and megakaryocytic cells

Morphometric evaluation of the fetal rat liver after maternal dexamethasone treatment: effect on the maturation of erythroid and megakaryocytic cells

Author Oliveira Neves, Flavia Macedo de Autor UNIFESP Google Scholar
Paccola, Camila Cicconi Autor UNIFESP Google Scholar
Miraglia, Sandra Maria Autor UNIFESP Google Scholar
Cipriano, Ivone Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: During pregnancy, glucocorticoids are frequently used to accelerate fetal lung maturation in preterm delivery. However, prenatal administration of glucocorticoids has been shown to affect organs such as fetal liver, an important hematopoietic organ during fetal development.Objective: the aim of this study was to document the qualitative and quantitative changes in erythroid and megakaryocytic cell populations found in fetal livers as well as the hematology profile in neonates after maternal glucocorticoid treatment in rats.Methods: Pregnant female Wistar rats were treated with dexamethasone 21-phosphate from days 13 to 16 of gestation. On the 17th day of pregnancy, the fetuses were collected and their livers processed for light and transmission electron microscopy. Glycol methacrylate-embedded sections were stained with PAS to determine the erythroblast and megakaryocytic cell frequencies. Fetal liver pieces embedded in Spurr resin were analyzed by transmission electron microscopy for morphologic changes. A standard hematology profile was evaluated in neonatal rats.Results: in the fetuses from treated dams, the total cell number of erythroid cells in livers was significantly reduced compared to control fetuses (P < .001), but erythroblasts did not present ultrastructural abnormalities. the degree of maturation in the megakaryocyte series tended to be increased. in neonates, there were elevated numbers of nucleated RBCs (P = .002), along with a higher HCT and HGB (P = .02). in addition, the platelet concentration was also significantly increased (P < .007).Conclusion: These results suggest that maternal dexamethasone treatment has quantitative effects on erythroid and megakaryocytic cells in fetal liver and the neonatal hematology profile in rats.
Keywords Erythroblast
erythroblastic island
hematologic analysis
neonate
Language English
Sponsor Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2013-12-01
Published in Veterinary Clinical Pathology. Hoboken: Wiley-Blackwell, v. 42, n. 4, p. 483-489, 2013.
ISSN 0275-6382 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 483-489
Origin http://dx.doi.org/10.1111/vcp.12080
Access rights Closed access
Type Article
Web of Science ID WOS:000330242500013
URI http://repositorio.unifesp.br/handle/11600/37063

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