Hematopoietic stem cell expansion caused by a synthetic fragment of leptin

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dc.contributor.author Dias, Carol C. [UNIFESP]
dc.contributor.author Nogueira-Pedro, Amanda [UNIFESP]
dc.contributor.author Barbosa, Christiano M. V. [UNIFESP]
dc.contributor.author Ribeiro-Filho, Antonio C. [UNIFESP]
dc.contributor.author Wasinski, Frederick [UNIFESP]
dc.contributor.author Araujo, Ronaldo C. [UNIFESP]
dc.contributor.author Oliveira, Vani Xavier de
dc.contributor.author Miranda, Antonio [UNIFESP]
dc.contributor.author Paredes-Gamero, Edgar J. [UNIFESP]
dc.date.accessioned 2016-01-24T14:34:49Z
dc.date.available 2016-01-24T14:34:49Z
dc.date.issued 2013-12-01
dc.identifier http://dx.doi.org/10.1016/j.peptides.2013.09.012
dc.identifier.citation Peptides. New York: Elsevier B.V., v. 50, p. 24-27, 2013.
dc.identifier.issn 0196-9781
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/37044
dc.description.abstract Leptin is a cytokine that regulates food intake, energy expenditure and hematopoiesis. Based on the tridimensional structure of the human leptin molecule, six fragments have been synthesized, (Ac-Lep(23-47)-NH2, [LEP1]; Ac-Lep(48-71)-NH2, [LEP2]; Ac-Lep(72-88)-NH2, [LEP3]; Ac-Lep(92-115)-NH2, [LEP4], Ac-[Ser(117)]-Lep(116-140)-NH2, [LEP5] and Ac-Lep(141-164)-NH2, [LEP6]), and their effects on hematopoiesis were evaluated. the mice were treated with 1 mg/kg LEP5 for 3 days. the mature and primitive hematopoietic populations were quantified. We observed that the mature populations from the bone marrow and spleen were not affected by LEP5. However, the peptide caused at least a two-fold increase in the number of hematopoietic stem cells, the most primitive population of the bone marrow. Additionally, the number of granulocyte/macrophage colony-forming units produced by bone marrow cells in methylcellulose also increased by 40% after treatment with LEP5, and the leptin receptor was activated. These results show that the leptin fragment LEP5 is a positive modulator of the in vivo expansion of hematopoietic stem cells. (C) 2013 Elsevier Inc. All rights reserved. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 24-27
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Peptides
dc.rights Acesso restrito
dc.title Hematopoietic stem cell expansion caused by a synthetic fragment of leptin en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal do ABC (UFABC)
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210170 Santo Andre, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.sponsorshipID FAPESP: 2009/54869-2
dc.identifier.doi 10.1016/j.peptides.2013.09.012
dc.description.source Web of Science
dc.identifier.wos WOS:000327524000005



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