Hematopoietic stem cell expansion caused by a synthetic fragment of leptin

Hematopoietic stem cell expansion caused by a synthetic fragment of leptin

Author Dias, Carol C. Autor UNIFESP Google Scholar
Nogueira-Pedro, Amanda Autor UNIFESP Google Scholar
Barbosa, Christiano M. V. Autor UNIFESP Google Scholar
Ribeiro-Filho, Antonio C. Autor UNIFESP Google Scholar
Wasinski, Frederick Autor UNIFESP Google Scholar
Araujo, Ronaldo C. Autor UNIFESP Google Scholar
Oliveira, Vani Xavier de Google Scholar
Miranda, Antonio Autor UNIFESP Google Scholar
Paredes-Gamero, Edgar J. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal do ABC (UFABC)
Abstract Leptin is a cytokine that regulates food intake, energy expenditure and hematopoiesis. Based on the tridimensional structure of the human leptin molecule, six fragments have been synthesized, (Ac-Lep(23-47)-NH2, [LEP1]; Ac-Lep(48-71)-NH2, [LEP2]; Ac-Lep(72-88)-NH2, [LEP3]; Ac-Lep(92-115)-NH2, [LEP4], Ac-[Ser(117)]-Lep(116-140)-NH2, [LEP5] and Ac-Lep(141-164)-NH2, [LEP6]), and their effects on hematopoiesis were evaluated. the mice were treated with 1 mg/kg LEP5 for 3 days. the mature and primitive hematopoietic populations were quantified. We observed that the mature populations from the bone marrow and spleen were not affected by LEP5. However, the peptide caused at least a two-fold increase in the number of hematopoietic stem cells, the most primitive population of the bone marrow. Additionally, the number of granulocyte/macrophage colony-forming units produced by bone marrow cells in methylcellulose also increased by 40% after treatment with LEP5, and the leptin receptor was activated. These results show that the leptin fragment LEP5 is a positive modulator of the in vivo expansion of hematopoietic stem cells. (C) 2013 Elsevier Inc. All rights reserved.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2009/54869-2
Date 2013-12-01
Published in Peptides. New York: Elsevier B.V., v. 50, p. 24-27, 2013.
ISSN 0196-9781 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 24-27
Origin http://dx.doi.org/10.1016/j.peptides.2013.09.012
Access rights Closed access
Type Article
Web of Science ID WOS:000327524000005
URI http://repositorio.unifesp.br/handle/11600/37044

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