Dengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazil

Dengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazil

Author Colombo, Tatiana Elias Google Scholar
Vedovello, Danila Google Scholar
Araki, Carlos Shigueru Google Scholar
Cogo-Moreira, Hugo Autor UNIFESP Google Scholar
Penha dos Santos, Izalco Nuremberg Google Scholar
Negri Reis, Andreia Francesli Google Scholar
Costa, Fabiana Rodrigues Google Scholar
Arao Antonio Cruz, Lilian Elisa Google Scholar
Casagrande, Liane Google Scholar
Regatieri, Livia Jose Google Scholar
Ferreira Junior, Jurandir Google Scholar
Morais Bronzoni, Roberta Vieira de Google Scholar
Schmidt, Diane J. Google Scholar
Nogueira, Mauricio Lacerda Google Scholar
Institution Fac Med Sao Jose do Rio Preto FAMERP
Univ Estadual Paulista Julio de Mesquitta & Filho
Universidade Federal de São Paulo (UNIFESP)
Prefeitura Sao Jose do Rio Preto
Univ Fed Mato Grosso
Tufts Univ
Abstract Background: Dengue is a serious public health problem in numerous countries. the ability to rapidly diagnosis dengue is important for patient triage and management. Detection of dengue viral protein, NS1, represents a new approach to dengue diagnosis.Objective: the present study aims to evaluate if there are false negative results using the NS1 Ag rapid assay (Panbio (R) Dengue Early ELISA) in two different epidemiological situations (epidemic and non-epidemic).Study design: 220 serum samples from patients with clinical symptoms of classical dengue fever were tested by NS1 antigen capture ELISA and Multiplex-Nested-PCR.Results: in samples collected in a non-epidemic period we found a 100% agreement of ELISA and RTPCR in dengue negative samples and 85% agreement of ELISA and RT-PCR in dengue positive samples. But when we tested samples during an epidemic period (large DENV-4 outbreak) we found 15% false negative results (p<0.05) in dengue negative samples.Conclusions: Due to false negative results for DENV-4, the sole use of the Panbio Dengue Early ELISA assay as a screening method for monitoring circulating dengue serotypes must be reevaluated. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Dengue
NS1 Ag rapid assay
False negative results
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2012/11733-6
FAPESP: 2011/10458-9
Date 2013-12-01
Published in Journal of Clinical Virology. Amsterdam: Elsevier B.V., v. 58, n. 4, p. 710-712, 2013.
ISSN 1386-6532 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 710-712
Origin http://dx.doi.org/10.1016/j.jcv.2013.10.021
Access rights Closed access
Type Article
Web of Science ID WOS:000327820600019
URI http://repositorio.unifesp.br/handle/11600/37040

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