Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates

Different Assay Conditions for Detecting the Production and Release of Heat-Labile and Heat-Stable Toxins in Enterotoxigenic Escherichia coli Isolates

Author Rocha, Leticia B. Google Scholar
Ozaki, Christiane Y. Google Scholar
Horton, Denise S. P. Q. Google Scholar
Menezes, Caroline A. Google Scholar
Silva, Anderson Google Scholar
Fernandes, Irene Google Scholar
Magnoli, Fabio C. Google Scholar
Vaz, Tania M. I. Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Piazza, Roxane M. F. Google Scholar
Institution Butantan Inst
São Paulo Trop Med Inst
Fleury Med & Hlth
Adolfo Lutz Inst
Universidade Federal de São Paulo (UNIFESP)
Abstract Enterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one or a few ETEC isolates to define the production and release of these toxins, which hinders the detection of ETEC by phenotypic approaches. Thus, the present study was undertaken to obtain a better understanding of ST and LT toxin production and release under laboratory conditions. Accordingly, a collection of 90 LT-, ST-, and ST/LT-producing ETEC isolates was used to determine a protocol for toxin production and release aimed at ETEC detection. for this, we used previously raised anti-LT antibodies and the anti-ST monoclonal and polyclonal antibodies described herein. the presence of bile salts and the use of certain antibiotics improved ETEC toxin production/release. Triton X-100, as chemical treatment, proved to be an alternative method for toxin release. Consequently, a common protocol that can increase the production and release of LT and ST toxins could facilitate and enhance the sensitivity of diagnostic tests for ETEC using the raised and described antibodies in the present work.
Keywords ETEC
heat-labile toxin
heat-stable toxin
production
release
detection
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-12-01
Published in Toxins. Basel: Mdpi Ag, v. 5, n. 12, p. 2384-2402, 2013.
ISSN 2072-6651 (Sherpa/Romeo, impact factor)
Publisher Mdpi Ag
Extent 2384-2402
Origin http://dx.doi.org/10.3390/toxins5122384
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000332792300007
URI http://repositorio.unifesp.br/handle/11600/37019

Show full item record




File

Name: WOS000332792300007.pdf
Size: 351.6Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account