Systematic review and new insights into the molecular characterization of the Candida rugosa species complex

Systematic review and new insights into the molecular characterization of the Candida rugosa species complex

Author Padovan, Ana Carolina Barbosa Autor UNIFESP Google Scholar
Melo, Analy Salles de Azevedo Autor UNIFESP Google Scholar
Colombo, Arnaldo Lopes Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Recently, Candida rugosa was characterized as a species complex comprising four taxa: C rugosa sensu stricto, Candida pseudorugosa, Candida neorugosa and Candida mesorugosa. Although considered relatively rare, several clusters of candidemia due to C rugosa complex had been reported presenting mortality rates close to 70%. in this work we discuss the systematization, phenotyping and molecular methods based on internal transcribed spacer region (ITS) sequencing and proteomic analyses for species identification, as well as clinical aspects of the C rugosa complex. We performed a Bayesian phylogenetic analysis using 72 ITS sequences representative of C rugosa complex isolates and related species within the genus. Biochemical, morphological and MALDI-TOF MS analyses were processed with C rugosa complex type strains and related species isolates. We described that the phylogeny showed four distinct clades inferred with high posterior probabilities, corresponding to the four species within the C. rugosa complex, excluding C. pararugosa. Biochemical and morphological aspects distinguished only C rugosa sensu strict but were not sufficient to accurately identify species within the rest of the complex. Protein spectrum profiles differentiated all reference strains from different species analyzed. To our knowledge, we presented the first phylogenetic analysis using a large collection of ITS sequences as well as proteomic profiles generated from isolates of the C rugosa complex and related species that can enlighten systematics, diagnostics and clinical research fields. (C) 2013 Elsevier Inc. All rights reserved.
Keywords Candida rugosa species complex
Candida pseudorugosa
Candida neorugosa
Candida mesorugosa
ITS sequencing
Proteomic profiles
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2007/08575-1
FAPESP: 2009/10155-6
CAPES: PNPD 02640-09-0
Date 2013-12-01
Published in Fungal Genetics and Biology. San Diego: Academic Press Inc Elsevier Science, v. 61, p. 33-41, 2013.
ISSN 1087-1845 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 33-41
Origin http://dx.doi.org/10.1016/j.fgb.2013.10.007
Access rights Open access Open Access
Type Review
Web of Science ID WOS:000327674300005
URI http://repositorio.unifesp.br/handle/11600/36997

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