Keratinized versus non-keratinized preputial flap onlay urethroplasty: does it make any difference in the histological analysis? An experimental study in rabbits

Keratinized versus non-keratinized preputial flap onlay urethroplasty: does it make any difference in the histological analysis? An experimental study in rabbits

Author Costa, Marnio Autor UNIFESP Google Scholar
Leslie, Bruno Autor UNIFESP Google Scholar
Rondon, Atila Autor UNIFESP Google Scholar
Bacelar, Herick Autor UNIFESP Google Scholar
Mattos, Ricardo Autor UNIFESP Google Scholar
Barbosa, Bruno Autor UNIFESP Google Scholar
Delcelo, Rosana Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Macedo, Antonio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: To compare the histological characteristics of keratinized versus non-keratinized onlay island flaps in an experimental rabbit model.Materials and Methods: Sixteen male rabbits were randomly allocated into two experimental groups: keratinized and non-keratinized onlay island flaps. A defect was created in the ventral aspect of the penile urethra. in the keratinized group, a longitudinal island flap was harvested from the external prepuce and rotated to cover the urethral defect. in the non-keratinized group a transverse island flap was harvested from the inner prepuce. the animals were sacrificed after 2, 4, 8 and 12 weeks.Results: the flaps were viable in all animals, and no deaths were associated with the procedure. Two urethrocutaneous fistulas were identified, one in each experimental group. A similar pattern of fibrosis was identified in both groups. the keratinized epithelium of the external prepuce kept its histological aspect and keratin production. Both keratinized and non-keratinized groups presented a slight decrease on the epithelial thickness, however without a statistically significant difference between groups.Conclusions: in this short-term rabbit model, we observed that the stratified squamous keratinized epithelium from the external prepuce kept its keratin production. There was no statistical influence of the flap type on the mean epithelial thickness.
Keywords Hypospadias
Surgical Flaps
Foreskin
Keratins
Urethra
Language English
Date 2013-11-01
Published in International Braz J Urol. Rio de Janeiro: Brazilian Soc Urol, v. 39, n. 6, p. 884-892, 2013.
ISSN 1677-5538 (Sherpa/Romeo, impact factor)
Publisher Brazilian Soc Urol
Extent 884-892
Origin http://dx.doi.org/10.1590/S1677-5538.IBJU.2013.06.16
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000329979600016
SciELO ID S1677-5538.IBJU.2013.06.16 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/36916

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