Fucan effect on CHO cell proliferation and migration

Fucan effect on CHO cell proliferation and migration

Autor Nobre, Leonardo Thiago Duarte Barreto Autor UNIFESP Google Scholar
Vidal, Arthur Anthunes Jacome Google Scholar
Almeida-Lima, Jailma Google Scholar
Oliveira, Ruth Medeiros Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Medeiros, Valquiria Pereira Autor UNIFESP Google Scholar
Trindade, Edvaldo da Silva Autor UNIFESP Google Scholar
Franco, Celia Regina Cavichiolo Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Rocha, Hugo Alexandre Oliveira Autor UNIFESP Google Scholar
Instituição Univ Fed Rio Grande do Norte
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Parana
Resumo Fucan is a term used to denominate sulfated L-fucose rich polysaccharides. Here, a heterofucan, named fucan B, was extracted from the Spatoglossum schroederi seaweed. This 21.5 kDa galactofucan inhibited CHO-Kl proliferation and migration when fibronectin was the substrate. Fucan B derivatives revealed that such effects depend on their degree of sulfation. Fucan B did not induce cell death, but promoted G1 cell cycle arrest. Western blotting and flow cytometry analysis suggest that fucan B binds to fibronectin and activates integrin, mainly integrin alpha 5 beta 1, which induces FAK/RAS/MEK/ERK activation. FAK activation inhibits CHO-K1 migration on fibronectin and ERK blocks cell cycle progression. This study indicates that fucan B could be applied in developing new antitumor drugs. (C) 2013 Elsevier B.V. All rights reserved.
Assunto Fucoidan
Antiproliferative
Cancer
MAPK pathway
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
MCT
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
FAPERN
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data 2013-10-15
Publicado em Carbohydrate Polymers. Oxford: Elsevier B.V., v. 98, n. 1, p. 224-232, 2013.
ISSN 0144-8617 (Sherpa/Romeo, fator de impacto)
Editor Elsevier B.V.
Extensão 224-232
Fonte http://dx.doi.org/10.1016/j.carbpol.2013.05.040
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000325835600030
URI http://repositorio.unifesp.br/handle/11600/36878

Mostrar registro completo




Arquivos deste item

Nome: WOS000325835600030.pdf
Tamanho: 2.029Mb
Formato: PDF
Descrição:
Visualizar/Abrir

Este item aparece na(s) seguinte(s) coleção(s)