Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus

Sleep deprivation reduces the lymphocyte count in a non-obese mouse model of type 1 diabetes mellitus

Autor Ruiz, Francieli Silva Autor UNIFESP Google Scholar
Andersen, Monica Levy Autor UNIFESP Google Scholar
Zager, Adriano Autor UNIFESP Google Scholar
Martins, Raquel Cristina Silva Autor UNIFESP Google Scholar
Tufik, Sergio Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo The objective of the present study was to determine whether sleep deprivation (SD) would promote changes in lymphocyte numbers in a type 1 diabetes model (non-obese diabetic, NOD, mouse strain) and to determine whether SD would affect female and male NOD compared to Swiss mice. The number of lymphocytes in peripheral blood after 24 and 96 h of SD (by multiple platform method) or equivalent period of time in home-cage controls was examined prior to the onset of diabetes. SD for 96 h significantly reduced lymphocytes in male Swiss mice compared to control (8.6 ± 2.1 vs 4.1 ± 0.7 10³/µL; P < 0.02). In male NOD animals, 24- and 96-h SD caused a significant decrease of lymphocytes compared to control (4.4 ± 0.3 vs 1.6 ± 0.5; P < 0.001 and 4.4 ± 0.3 vs 0.9 ± 0.1 10³/µL; P < 0.00001, respectively). Both 24- and 96-h SD induced a reduction in the number of lymphocytes in female Swiss (7.5 ± 0.5 vs 4.5 ± 0.5, 4.4 ± 0.6 10³/µL; P < 0.001, respectively) and NOD mice (4 ± 0.6 vs 1.8 ± 0.2, 1.2 ± 0.4 10³/µL; P < 0.01, respectively) compared to the respective controls. Loss of sleep induced lymphopenia in peripheral blood in both genders and strains used. Since many cases of autoimmunity present reduced numbers of lymphocytes and, in this study, it was more evident in the NOD strain, our results suggest that SD should be considered a risk factor in the onset of autoimmune disorders.
Palavra-chave Sleep deprivation
Stress
Lymphopenia
Diabetes
Non-obese diabetic mouse
Swiss mice
Idioma Inglês
Data de publicação 2007-05-01
Publicado em Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 40, n. 5, p. 633-637, 2007.
ISSN 0100-879X (Sherpa/Romeo, fator de impacto)
Publicador Associação Brasileira de Divulgação Científica
Extensão 633-637
Fonte http://dx.doi.org/10.1590/S0100-879X2006005000086
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000246576900005
SciELO S0100-879X2007000500005 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/3687

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