Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranes

Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow-fiber membranes

Autor Borsoi-Ribeiro, Mariana Google Scholar
Lazzarotto Bresolin, Igor Tadeu Autor UNIFESP Google Scholar
Vijayalakshmi, Mookambeswaran Google Scholar
Alves Bueno, Sonia Maria Google Scholar
Instituição Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Univ Technol Compiegne
VIT Univ Vellore
Resumo Iminodiacetic acid (IDA) and tris(2-aminoethyl)amine (TREN) chelating ligands were immobilized on poly(ethylene vinyl alcohol) (PEVA) hollow-fiber membranes after activation with epichlorohydrin or butanediol diglycidyl ether (bisoxirane). the affinity membranes complexed with Cu(II) were evaluated for adsorption of human immunoglobulin G (IgG). the effects of matrix activation and buffer system on adsorption of IgG were studied. Isotherms of batch IgG adsorption onto finely cut membranes showed that neither of the chelates, IDA-Cu(II) or TREN-Cu(II), had a Langmuirean behavior with negative cooperativity for IgG binding. A comparison of equilibrium and dynamic maximum capacities showed that the dynamic capacity for a mini-cartridge in a cross-flow filtration mode (52.5 and 298.4mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively) was somewhat higher than the equilibrium capacity (9.2 and 73.3mgg(-1) dry weight for PEVA-TREN-Cu(II) and PEVA-IDA-Cu(II), respectively). When mini-cartridges were used, the dynamic adsorption capacity of IDA-Cu(II) was the same for both mini-cartridge and agarose gel. Copyright (c) 2013 John Wiley & Sons, Ltd.
Assunto human IgG
IMAC
Cu(II)
tris(2-aminoethyl)amine
affinity membranes
adsorption
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data 2013-10-01
Publicado em Journal of Molecular Recognition. Hoboken: Wiley-Blackwell, v. 26, n. 10, p. 514-520, 2013.
ISSN 0952-3499 (Sherpa/Romeo, fator de impacto)
Editor Wiley-Blackwell
Extensão 514-520
Fonte http://dx.doi.org/10.1002/jmr.2296
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000323674900010
URI http://repositorio.unifesp.br/handle/11600/36790

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