The influence of orexins on ethanol-induced behavioral sensitization in male mice

The influence of orexins on ethanol-induced behavioral sensitization in male mice

Author Macedo, Giovana Camila Autor UNIFESP Google Scholar
Kawakami, Suzi Emiko Autor UNIFESP Google Scholar
Vignoli, Thiago Autor UNIFESP Google Scholar
Sinigaglia-Coimbra, Rita Autor UNIFESP Google Scholar
Suchecki, Deborah Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Recent evidence indicates the involvement of orexin in reward circuitry and drug addiction. in the present study we evaluated the role of orexin in ethanol-induced behavioral sensitization. in the first experiment, Swiss male mice received seven administrations of saline or ethanol (2.2 g/kg, i.p., chronic), every other day. On the last day of treatment, half of saline-treated mice received a saline injection (saline) whereas the other half received 2.2 g/kg of ethanol (i.p., acute). Behavioral sensitization was assessed by locomotor activity tests and after the last one, immunoreactivity for orexin and Fos (ORX+Fos-ir) was assessed in the lateral hypothalamic area. Chronic ethanol treatment produced behavioral sensitization and a trend for greater ORX + Fos-ir. in the second experiment, mice were treated as in Experiment 1 and type 1 orexin receptor antagonist, SB334867 (20 mg/kg), was administered before the ethanol challenge successfully blocking the expression of sensitization in mice chronically treated with EtOH. These results indicate that orexin plays a role in ethanol-induced behavioral sensitization. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
Keywords Ethanol
Behavioral sensitization
Orexin type 1 receptor
Language English
Sponsor Associacao Fundo de Incentivo a Pesquisa (AFIP)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 98-14303-3
Date 2013-09-13
Published in Neuroscience Letters. Clare: Elsevier B.V., v. 551, p. 84-88, 2013.
ISSN 0304-3940 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 84-88
Access rights Closed access
Type Article
Web of Science ID WOS:000324227800018

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