FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss

FAS and FAS-L Genotype and Expression in Patients With Recurrent Pregnancy Loss

Author Andrade Banzato, Priscilla Chamelete Autor UNIFESP Google Scholar
Daher, Silvia Autor UNIFESP Google Scholar
Traina, Evelyn Autor UNIFESP Google Scholar
Torloni, Maria Regina Autor UNIFESP Google Scholar
Gueuvoghlanian-Silva, Barbara Yasmin Autor UNIFESP Google Scholar
Puccini, Renata Fiorini Autor UNIFESP Google Scholar
Tezotto Pendeloski, Karen Priscilla Autor UNIFESP Google Scholar
Mattar, Rosiane Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We assessed FAS and FAS-L gene polymorphisms and messenger RNA (mRNA) levels in patients with recurrent pregnancy loss (RPL). This case-control study compared 129 women with RPL with 235 healthy multiparous women (control group). Genomic DNA and total mRNA were extracted from whole blood, and polymorphisms genotyping was performed by polymerase chain reaction (PCR). Messenger RNA expression levels were analyzed by real-time PCR. Data were analyzed by chi-square and Fisher exact tests; P < .05 was considered significant. There were no significant differences in the FAS (670 A/G) genotype or allelic frequencies between the RPL and control groups. We found significant differences in the FAS-L (844 C/T) genotype and allelic frequencies between women with RPL and controls. Patients with RPL had significantly higher FAS-L expression. Our data suggest that FAS-L gene polymorphism is associated with increased susceptibility to RPL. Moreover, women with RPL seem to abnormally express FAS-FAS-L molecules.
Keywords recurrent pregnancy loss
polymorphism
FAS
FAS-L
apoptosis
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 09/50236-5
CNPq: 301886/2009-1
Date 2013-09-01
Published in Reproductive Sciences. Thousand Oaks: Sage Publications Inc, v. 20, n. 9, p. 1111-1115, 2013.
ISSN 1933-7191 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Inc
Extent 1111-1115
Origin http://dx.doi.org/10.1177/1933719113477488
Access rights Closed access
Type Article
Web of Science ID WOS:000323180300015
URI http://repositorio.unifesp.br/handle/11600/36735

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