Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples

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dc.contributor.author Morais Sana, Dandara Emery
dc.contributor.author Miranda, Priscila Mayrink de
dc.contributor.author Vieira Pitol, Bruna Caroline
dc.contributor.author Moran, Mariana Soares
dc.contributor.author Toledo Silva, Nayara Nascimento
dc.contributor.author Cotrim Guerreiro da Silva, Ismael Dali [UNIFESP]
dc.contributor.author Stocco, Rita de Cassia
dc.contributor.author Becak, Willy
dc.contributor.author Lima, Angelica Alves
dc.contributor.author Carneiro, Claudia Martins
dc.date.accessioned 2016-01-24T14:34:20Z
dc.date.available 2016-01-24T14:34:20Z
dc.date.issued 2013-09-01
dc.identifier http://dx.doi.org/10.1002/dc.22955
dc.identifier.citation Diagnostic Cytopathology. Hoboken: Wiley-Blackwell, v. 41, n. 9, p. 785-792, 2013.
dc.identifier.issn 8755-1039
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36687
dc.description.abstract Herein, we evaluated cervical samples from normal tissue or HPV-infected tissue, to determine if the relative nuclear/cytoplasmic ratio (NA/CA) and the presence of nonclassical cytological criteria are a novel cytological criterion for the diagnosis of HPV. Significantly, larger NA/CA ratios were found for the HPV-ATYPIA+ and HPV+ATYPIA+ groups compared with HPV-ATYPIA- group, regardless of collection method. for the samples collected with a spatula, only three samples from the HPV-ATIPIA- group showed four or more nonclassical parameters (i.e., were positive), while a larger number of the samples in the HPV-ATYPIA+, HPV+ATYPIA-, and HPV+ATYPIA+ groups were positive (13, 4, and 13 samples, respectively). Among those collected with a brush, no sample showed four or more nonclassical criteria in the HPV-ATYPIA- group, while a number of samples were positive in the HPV-ATYPIA+, HPV+ATYPIA-, and HPV+ATYPIA+ groups (4, 3, and 4 samples, respectively). HPV infection was associated with significant morphometrical changes; no increase in the NA/CA ratio was found in the HPV+ATYPIA- samples, compared with the HPV-ATIPIA- samples collected with either a spatula or a brush. in conclusion, by including nonclassical cytological criteria into the patient diagnosis, we were able to reduce the number of false negative and false positive HPV diagnoses made using conventional cytology alone. Diagn. Cytopathol. 2013;41:785-792. (c) 2013 Wiley Periodicals, Inc. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Universidade Federal de Ouro Preto, Brazil
dc.format.extent 785-792
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Diagnostic Cytopathology
dc.rights Acesso restrito
dc.subject HPV en
dc.subject cervix-vaginal cytology en
dc.subject morphometry en
dc.subject nonclassical criteria en
dc.subject molecular biology en
dc.title Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Univ Fed Ouro Preto
dc.contributor.institution Inst Butantan
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Ouro Preto, Dept Anal Clin, Escola Farm, Ouro Preto, MG, Brazil
dc.description.affiliation Inst Butantan, Genet Lab, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo UNIFESP, Dept Ginecol, São Paulo, Brazil
dc.description.affiliation Univ Fed Ouro Preto, Lab Imunopatol, Nucleo Pesquisas Ciencias Biol, ICEB 2, Ouro Preto, MG, Brazil
dc.description.affiliation Univ Fed Ouro Preto, Lab Pesquisas Clin, CiPharma Programa Posgrad Ciencias Farmaceut, Escola Farm, Ouro Preto, MG, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo UNIFESP, Dept Ginecol, São Paulo, Brazil
dc.identifier.doi 10.1002/dc.22955
dc.description.source Web of Science
dc.identifier.wos WOS:000323410600006



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