hTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates

hTERT and TP53 deregulation in intestinal-type gastric carcinogenesis in non-human primates

Author Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Calcagno, Danielle Queiroz Autor UNIFESP Google Scholar
Khayat, Andre Salim Google Scholar
Raiol Silva, Tanielly Cristina Google Scholar
Pereira Carneiro Muniz, Jose Augusto Google Scholar
Assumpcao, Paulo Pimentel Google Scholar
Cardoso Smith, Marilia de Arruda Autor UNIFESP Google Scholar
Burbano, Rommel Rodriguez Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Minist Saude
Abstract Despite the high incidence, the molecular events involved in intestinal-type gastric carcinogenesis remains unclear. We previously established an intestinal-type gastric carcinogenesis model in Cebus apella, a New World monkey. in the present study, we evaluated hTERT and TP53 mRNA expression, as well as their protein immunoreactivity, in normal mucosa, non-atrophic gastritis, atrophic gastritis, intestinal metaplasia, and intestinal-type gastric cancer samples of non-human primates treated with N-methyl-nitrosourea. in addition, we evaluated the number of TP53 copies in these samples. Although hTERT immunoreactivity was only detected in gastric cancer, a continuous increase of hTERT mRNA expression was observed from non-atrophic gastritis to gastric tumors. No sample presented p53 immunoreactivity. However, we also observed a continuous decrease of TP53 mRNA expression during the sequential steps of gastric carcinogenesis. Moreover, loss of TP53 copies was observed in intestinal metaplasia and gastric cancer samples. Our study highlights that hTERT and TP53 have a key role in intestinal-type gastric cancer initiation.
Keywords hTERT
TP53
Gastric carcinogenesis
Non-human primates
Precancerous lesions
Animal model
Language English
Date 2013-08-01
Published in Clinical and Experimental Medicine. Milan: Springer-verlag Italia Srl, v. 13, n. 3, p. 221-224, 2013.
ISSN 1591-8890 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 221-224
Origin http://dx.doi.org/10.1007/s10238-012-0195-4
Access rights Closed access
Type Letter
Web of Science ID WOS:000322677400009
URI http://repositorio.unifesp.br/handle/11600/36584

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