Antiangiogenic activity and direct antitumor effect from a sulfated polysaccharide isolated from seaweed

Antiangiogenic activity and direct antitumor effect from a sulfated polysaccharide isolated from seaweed

Author Guerra Dore, Celina Maria P. Google Scholar
Faustino Alves, Monique Gabriela C. Google Scholar
Santos, Nednaldo D. Google Scholar
Cruz, Ana Katarina M. Google Scholar
Camara, Rafael Barros G. Autor UNIFESP Google Scholar
Castro, Allisson Jonathan G. Google Scholar
Alves, Luciana Guimaraes Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Leite, Edda Lisboa Google Scholar
Institution Fed Univ Rio Grande Norte UFRN
Universidade Federal de São Paulo (UNIFESP)
Abstract Angiogenesis is a dynamic proliferation and differentiation process. It requires endothelial proliferation, migration and tube formation. in this context, endothelial cells are a preferred target for several studies and therapies. Anionic polysaccharides (SV1 and PSV1) from brown seaweed Sargassum vulgare were fractionated (SV1), purified (PSV1) and displayed with high total sugars, sulfate content and very low level of protein. the antiangiogenic efficacy of polysaccharides was examined in vivo in the chick chorioallantoic membrane (CAM) model by using fertilized eggs. Decreases in the density of the capillaries were assessed and scored. the results showed that SV1 and PSV1 have an inhibitory effect on angiogenesis. These results were also confirmed by the inhibition of tubulogenesis in rabbit aorta endothelial cell (RAEC) in matrigel. These compounds were assessed in an apoptosis assay (Annexin V-FITC/PI) and cell viability by MTT assay of RAEC. These polysaccharides did not affect the viability and did not have apoptotic or necrotic action. RAEC cell when incubated with SV1 and PSV1 showed inhibition of VEGF secretion, observed when compounds were incubated at 25,50 and 100 mu g/mu L.. the VEGF secretion with the RAEC cell line for 24 h was more effective for PSV1 at 50 mu g/mu L (71.4%) than for SV1 at 100 mu g/mu L (75.9%). SV1 and PSV1 had an antiproliferative action (47%) against tumor cell line HeLa. Our results indicate that these sulfated polysaccharides have antiangiogenic and antitumor actions. (C) 2013 Elsevier Inc. All rights reserved.
Language English
Date 2013-07-01
Published in Microvascular Research. San Diego: Academic Press Inc Elsevier Science, v. 88, p. 12-18, 2013.
ISSN 0026-2862 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 12-18
Origin http://dx.doi.org/10.1016/j.mvr.2013.03.001
Access rights Closed access
Type Article
Web of Science ID WOS:000326007600002
URI http://repositorio.unifesp.br/handle/11600/36495

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