Fish oil attenuates methylmalonate-induced seizures

Fish oil attenuates methylmalonate-induced seizures

Author Reschke Bandero, Cristina Ruedell Google Scholar
Salvadori, Mirian G. S. S. Google Scholar
Gomes, Anajara Teixeira Google Scholar
Dal Ri, Nadja M. K. Google Scholar
Furian, Ana Flavia Google Scholar
Oliveira, Mauro Schneider Google Scholar
Rambo, Leonardo Magno Google Scholar
Scorza, Fulvio A. Autor UNIFESP Google Scholar
Cysneiros, Roberta M. Google Scholar
Emanuelli, Tatiana Google Scholar
Mello, Carlos Fernando Google Scholar
Institution Universidade Federal de Sergipe (UFS)
Universidade Federal de São Paulo (UNIFESP)
Univ Presbiteriana Mackenzie
Abstract Methylmalonic acidemias are inherited metabolic disorders characterized by methylmalonate (MMA) accumulation and neurological dysfunction, including seizures. Dietary fatty acids are known as an important energy source and reduce seizure activity in selected acute animal models. This study investigated whether chronic treatment with fish oil or with oleic acid attenuates MMA-induced seizures and whether maintenance of Na+,K+-ATPase activity was involved in such an effect. Adult male Wistar rats were given fish oil (85 mg/kg), oleic acid (85 mg/kg) or vehicle (0.42% aqueous Cremophor EL (TM), 4 mL/kg/body weight/day), p.o., for 75 days. On the 73th day a cannula was implanted in the right lateral ventricle with electrodes over the parietal cortex for EEG recording. On the 76th day the animals were injected with NaCl (2.5 mu Lmol/2.5 mu L, i.c.v.), or with MMA (2.5 mu mol/2.5 mu L, i.c.v.), and seizure activity was measured by electroencephagraphic (EEG) recording with concomitant behavior monitoring. the effect of prostaglandin E-2 (PGE(2)) on Na+,K+-ATPase activity of slices of cerebral cortex from NaCl-injected animals was determined. Fish oil increased the latency to MMA-induced tonic-clonic seizures, reduced the mean amplitude of ictal EEG recordings, and prevented PGE(2)-induced decrease of Na+,K+-ATPase activity in cortical slices in vitro. Oleic acid decreased mean amplitude of ictal EEG recordings. the results support that fish oil decreases MMA-induced seizures. the decreased sensitivity of Na+,K+-ATPase to the inhibitory effect of PGE(2) in fish oil-treated animals may be related to the currently reported anticonvulsant activity. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Seizure
Methylmalonate
Fatty acid
Fish oil
Oleic acid
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number CNPq: 476551/2009-9
CNPq: 552898/2008-2
CNPq: 141164/2010-7
CNPq: 150905/2009-2
CNPq: 301552/2007-0
Date 2013-07-01
Published in Epilepsy Research. Amsterdam: Elsevier B.V., v. 105, n. 1-2, p. 69-76, 2013.
ISSN 0920-1211 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 69-76
Origin http://dx.doi.org/10.1016/j.eplepsyres.2013.01.005
Access rights Closed access
Type Article
Web of Science ID WOS:000320737500008
URI http://repositorio.unifesp.br/handle/11600/36481

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