Structural characterization of coagulant Moringa oleifera Lectin and its effect on hemostatic parameters

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dc.contributor.author Luz, Luciana de Andrade
dc.contributor.author Silva, Mariana Cristina Cabral [UNIFESP]
dc.contributor.author Ferreira, Rodrigo da Silva [UNIFESP]
dc.contributor.author Santana, Lucimeire Aparecida de [UNIFESP]
dc.contributor.author Silva-Luccao, Rosemeire Aparecida
dc.contributor.author Mentele, Reinhard
dc.contributor.author Oliva, Maria Luiza Vilela [UNIFESP]
dc.contributor.author Paiva, Patricia Maria Guedes [UNIFESP]
dc.contributor.author Coelho, Luana Cassandra Breitenbach Barroso
dc.date.accessioned 2016-01-24T14:31:54Z
dc.date.available 2016-01-24T14:31:54Z
dc.date.issued 2013-07-01
dc.identifier http://dx.doi.org/10.1016/j.ijbiomac.2013.03.044
dc.identifier.citation International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 58, p. 31-36, 2013.
dc.identifier.issn 0141-8130
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36442
dc.description.abstract Lectins are carbohydrate recognition proteins. cMoL, a coagulant Moringa oleifera Lectin, was isolated from seeds of the plant. Structural studies revealed a heat-stable and pH resistant protein with 101 amino acids, 11.67 theoretical pI and 81% similarity with a M. oleifera flocculent protein. Secondary structure content was estimated as 46% alpha-helix, 12% beta-sheets, 17% beta-turns and 25% unordered structures belonging to the alpha/beta tertiary structure class. cMoL significantly prolonged the time required for blood coagulation, activated partial thromboplastin (aPTF) and prothrombin times (PT), but was not so effective in prolonging aPTT in asialofetuin presence. cMoL acted as an anticoagulant protein on in vitro blood coagulation parameters and at least on aPTT, the lectin interacted through the carbohydrate recognition domain. (C) 2013 Elsevier B.V. All rights reserved. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent 31-36
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof International Journal of Biological Macromolecules
dc.rights Acesso aberto
dc.subject Coagulant Moringa oleifera lectin en
dc.subject Primary sequence en
dc.subject Circular dichroism en
dc.subject Anticoagulant lectin and Hemostatic parameters en
dc.title Structural characterization of coagulant Moringa oleifera Lectin and its effect on hemostatic parameters en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de Pernambuco (UFPE)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Estadual Oeste Parana
dc.contributor.institution LMU
dc.contributor.institution Max Planck Inst Biochem
dc.description.affiliation Univ Fed Pernambuco, Dept Bioquim, BR-50670901 Recife, PE, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliation Univ Estadual Oeste Parana, Ctr Engn & Ciencias Exatas, BR-85903000 Toledo, PR, Brazil
dc.description.affiliation LMU, Inst Clin Neuroimmunol, Munich, Germany
dc.description.affiliation Max Planck Inst Biochem, Dept Prot Analyt, D-82152 Martinsried, Germany
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.identifier.file WOS000320746200006.pdf
dc.identifier.doi 10.1016/j.ijbiomac.2013.03.044
dc.description.source Web of Science
dc.identifier.wos WOS:000320746200006



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