Structural characterization of coagulant Moringa oleifera Lectin and its effect on hemostatic parameters

Structural characterization of coagulant Moringa oleifera Lectin and its effect on hemostatic parameters

Author Luz, Luciana de Andrade Google Scholar
Silva, Mariana Cristina Cabral Autor UNIFESP Google Scholar
Ferreira, Rodrigo da Silva Autor UNIFESP Google Scholar
Santana, Lucimeire Aparecida de Autor UNIFESP Google Scholar
Silva-Luccao, Rosemeire Aparecida Google Scholar
Mentele, Reinhard Google Scholar
Oliva, Maria Luiza Vilela Autor UNIFESP Google Scholar
Paiva, Patricia Maria Guedes Autor UNIFESP Google Scholar
Coelho, Luana Cassandra Breitenbach Barroso Google Scholar
Institution Universidade Federal de Pernambuco (UFPE)
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Oeste Parana
LMU
Max Planck Inst Biochem
Abstract Lectins are carbohydrate recognition proteins. cMoL, a coagulant Moringa oleifera Lectin, was isolated from seeds of the plant. Structural studies revealed a heat-stable and pH resistant protein with 101 amino acids, 11.67 theoretical pI and 81% similarity with a M. oleifera flocculent protein. Secondary structure content was estimated as 46% alpha-helix, 12% beta-sheets, 17% beta-turns and 25% unordered structures belonging to the alpha/beta tertiary structure class. cMoL significantly prolonged the time required for blood coagulation, activated partial thromboplastin (aPTF) and prothrombin times (PT), but was not so effective in prolonging aPTT in asialofetuin presence. cMoL acted as an anticoagulant protein on in vitro blood coagulation parameters and at least on aPTT, the lectin interacted through the carbohydrate recognition domain. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Coagulant Moringa oleifera lectin
Primary sequence
Circular dichroism
Anticoagulant lectin and Hemostatic parameters
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2013-07-01
Published in International Journal of Biological Macromolecules. Amsterdam: Elsevier B.V., v. 58, p. 31-36, 2013.
ISSN 0141-8130 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 31-36
Origin http://dx.doi.org/10.1016/j.ijbiomac.2013.03.044
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000320746200006
URI http://repositorio.unifesp.br/handle/11600/36442

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