Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks

Phylogenetic Analysis Reveals a High Prevalence of Sporothrix brasiliensis in Feline Sporotrichosis Outbreaks

Author Rodrigues, Anderson Messias Autor UNIFESP Google Scholar
Teixeira, Marcus de Melo Google Scholar
Hoog, G. Sybren de Google Scholar
Pacheco Schubach, Tania Maria Google Scholar
Pereira, Sandro Antonio Google Scholar
Fernandes, Geisa Ferreira Autor UNIFESP Google Scholar
Lopes Bezerra, Leila Maria Google Scholar
Felipe, Maria Sueli Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
CBS KNAW Fungal Biodivers Ctr
Universidade de Brasília (UnB)
Fundacao Oswaldo Cruz
Universidade do Estado do Rio de Janeiro (UERJ)
Univ Catolica Brasilia
Abstract Sporothrix schenckii, previously assumed to be the sole agent of human and animal sporotrichosis, is in fact a species complex. Recently recognized taxa include S. brasiliensis, S. globosa, S. mexicana, and S. luriei, in addition to S. schenckii sensu stricto. Over the last decades, large epidemics of sporotrichosis occurred in Brazil due to zoonotic transmission, and cats were pointed out as key susceptible hosts. in order to understand the eco-epidemiology of feline sporotrichosis and its role in human sporotrichosis a survey was conducted among symptomatic cats. Prevalence and phylogenetic relationships among feline Sporothrix species were investigated by reconstructing their phylogenetic origin using the calmodulin (CAL) and the translation elongation factor-1 alpha (EF1 alpha) loci in strains originated from Rio de Janeiro (RJ, n = 15), Rio Grande do Sul (RS, n = 10), Parana (PR, n = 4), São Paulo (SP, n = 3) and Minas Gerais (MG, n = 1). Our results showed that S. brasiliensis is highly prevalent among cats (96.9%) with sporotrichosis, while S. schenckii was identified only once. the genotype of Sporothrix from cats was found identical to S. brasiliensis from human sources confirming that the disease is transmitted by cats. Sporothrix brasiliensis presented low genetic diversity compared to its sister taxon S. schenckii. No evidence of recombination in S. brasiliensis was found by split decomposition or PHI-test analysis, suggesting that S. brasiliensis is a clonal species. Strains recovered in states SP, MG and PR share the genotype of the RJ outbreak, different from the RS clone. the occurrence of separate genotypes among strains indicated that the Brazilian S. brasiliensis epidemic has at least two distinct sources. We suggest that cats represent a major host and the main source of cat and human S. brasiliensis infections in Brazil.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: FAPESP 2011/07350-1
CAPES: BEX 2325/11-0
Date 2013-06-01
Published in Plos Neglected Tropical Diseases. San Francisco: Public Library Science, v. 7, n. 6, 14 p., 2013.
ISSN 1935-2735 (Sherpa/Romeo, impact factor)
Publisher Public Library Science
Extent 14
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000321201300038

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