Dragon's blood Croton palanostigma induces genotoxic effects in mice

Dragon's blood Croton palanostigma induces genotoxic effects in mice

Author Maistro, Edson Luis Google Scholar
Ganthous, Giulia Google Scholar
Machado, Marina da Silva Google Scholar
Zermiani, Tailyn Google Scholar
Andrade, Sergio Faloni de Google Scholar
Pires Rosa, Paulo Cesar Autor UNIFESP Google Scholar
Perazzo, Fabio Ferreira Autor UNIFESP Google Scholar
Institution Univ Estadual Paulista UNESP
Univ Vale Itajai UNIVALI
Universidade Federal de São Paulo (UNIFESP)
Abstract Ethnopharmacological relevance: Dragon's blood is a dark-red sap produced by species from the genus Croton (Euphorbiaceae), which has been used as a famous traditional medicine since ancient times in many countries, with scarce data about its safe use in humans. in this research, we studied genotoxicity and clastogenicity of Croton palanostigma sap using the comet assay and micronucleus test in cells of mice submitted to acute treatment.Material and methods: HPLC analysis was performed to identify the main components of the sap. the sap was administered by oral gavage at doses of 300 mg/kg, 1000 mg/kg and 2000 mg/kg. for the analysis, the comet assay was performed on the leukocytes and liver cells collected 24 h after treatment, and the micronucleus test (MN) on bone marrow cells. Cytotoxicity was assessed by scoring 200 consecutive polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio).Results and conclusion: the alkaloid taspine was the main compound indentified in the crude sap of Croton palanostigma. the results of the genotoxicity assessment show that all sap doses tested produced genotoxic effects in leukocytes and liver cells and also produced clastogenic/aneugenic effects in bone marrow cells of mice at the two higher doses tested. the PCE/NCE ratio indicated no cytotoxicity. the data obtained suggest caution in the use of Croton palanostigma sap by humans considering its risk of carcinogenesis. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
Keywords Croton palanostigma
Dragon's blood
Sangre de grado
Euphorbiaceae
Comet assay
Micronucleus test
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-05-20
Published in Journal of Ethnopharmacology. Clare: Elsevier B.V., v. 147, n. 2, p. 406-411, 2013.
ISSN 0378-8741 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 406-411
Origin http://dx.doi.org/10.1016/j.jep.2013.03.026
Access rights Closed access
Type Article
Web of Science ID WOS:000319180700018
URI http://repositorio.unifesp.br/handle/11600/36346

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