Antithrombin stabilisation by sulfated carbohydrates correlates with anticoagulant activity

Antithrombin stabilisation by sulfated carbohydrates correlates with anticoagulant activity

Author Lima, Marcelo A. Autor UNIFESP Google Scholar
Hughes, Ashley J. Google Scholar
Veraldi, Noemi Google Scholar
Rudd, Timothy R. Google Scholar
Hussain, Rohanah Google Scholar
Brito, Adriana S. Autor UNIFESP Google Scholar
Chavante, Suely F. Google Scholar
Tersariol, Ivarne I. Autor UNIFESP Google Scholar
Siligardi, Giuliano Google Scholar
Nader, Helena B. Autor UNIFESP Google Scholar
Yates, Edwin A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Diamond Light Source Ltd
Univ Liverpool
Ist Ric Chim & Biochim G Ronzoni
Univ Fed Rio Grande do Norte
Abstract Thermal stabilisation of native antithrombin-III (AT), determined using differential scanning fluorimetry, correlated with the anticoagulant activity of heparin and heparin-related saccharides. Similar conformational changes were induced in native AT by a variety of active and inactive heparin-related sulfated carbohydrates, measured in solution using synchrotron radiation circular dichroism, and their anticoagulant activities. Measurement of native AT stabilisation provides a convenient assay for prospective anticoagulants and represents an additional parameter by which to compare biosimilar heparins.
Language English
Sponsor National Science Foundation's National High Magnetic Field Laboratory User Program in the Advanced Magnetic Resonance Imaging and Spectroscopy (AMRIS) Facility (McKnight Brain Institute, University of Florida, USA)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Date 2013-05-01
Published in Medchemcomm. Cambridge: Royal Soc Chemistry, v. 4, n. 5, p. 870-873, 2013.
ISSN 2040-2503 (Sherpa/Romeo, impact factor)
Publisher Royal Soc Chemistry
Extent 870-873
Access rights Closed access
Type Article
Web of Science ID WOS:000318302500027

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