Expression and cellular trafficking of GP82 and GP90 glycoproteins during Trypanosoma cruzi metacyclogenesis

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dc.contributor.author Bayer-Santos, Ethel [UNIFESP]
dc.contributor.author Cunha-e-Silva, Narcisa Leal
dc.contributor.author Yoshida, Nobuko [UNIFESP]
dc.contributor.author Silveira, Jose Franco da [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:38Z
dc.date.available 2016-01-24T14:31:38Z
dc.date.issued 2013-05-01
dc.identifier http://dx.doi.org/10.1186/1756-3305-6-127
dc.identifier.citation Parasites & Vectors. London: Biomed Central Ltd, v. 6, 10 p., 2013.
dc.identifier.issn 1756-3305
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36246
dc.description.abstract Background: the transformation of noninfective epimastigotes into infective metacyclic trypomastigotes (metacyclogenesis) is a fundamental step in the life cycle of Trypanosoma cruzi, comprising several morphological and biochemical changes. GP82 and GP90 are glycoproteins expressed at the surface of metacyclic trypomastigote, with opposite roles in mammalian cell invasion. GP82 is an adhesin that promotes cell invasion, while GP90 acts as a negative regulator of parasite internalization. Our understanding of the synthesis and intracellular trafficking of GP82 and GP90 during metacyclogenesis is still limited. Therefore, we decided to determine whether GP82 and GP90 are expressed only in fully differentiated metacyclic forms or they start to be expressed in intermediate forms undergoing differentiation.Methods: Parasite populations enriched in intermediate forms undergoing differentiation were analyzed by quantitative real-time PCR, Western blot, flow cytometry and immunofluorescence to assess GP82 and GP90 expression.Results: We found that GP82 and GP90 mRNAs and proteins are expressed in intermediate forms and reach higher levels in fully differentiated metacyclic forms. Surprisingly, GP82 and GP90 presented distinct cellular localizations in intermediate forms compared to metacyclic trypomastigotes. in intermediate forms, GP82 is localized in organelles at the posterior region and colocalizes with cruzipain, while GP90 is localized at the flagellar pocket region.Conclusions: This study discloses new aspects of protein expression and trafficking during T. cruzi differentiation by showing that the machinery involved in GP82 and GP90 gene expression starts to operate early in the differentiation process and that different secretion pathways are responsible for delivering these glycoproteins toward the cell surface. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 10
dc.language.iso eng
dc.publisher Biomed Central Ltd
dc.relation.ispartof Parasites & Vectors
dc.rights Acesso aberto
dc.subject Trypanosoma cruzi en
dc.subject Metacyclogenesis en
dc.subject Surface proteins en
dc.subject Gene expression en
dc.subject Intracellular trafficking en
dc.title Expression and cellular trafficking of GP82 and GP90 glycoproteins during Trypanosoma cruzi metacyclogenesis en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Federal do Rio de Janeiro (UFRJ)
dc.description.affiliation Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliation Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, BR-21949900 Rio de Janeiro, RJ, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.file WOS000318849400001.pdf
dc.identifier.doi 10.1186/1756-3305-6-127
dc.description.source Web of Science
dc.identifier.wos WOS:000318849400001



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