Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection

Vaccination Using Recombinants Influenza and Adenoviruses Encoding Amastigote Surface Protein-2 Are Highly Effective on Protection against Trypanosoma cruzi Infection

Autor Alves Barbosa, Rafael Polidoro Google Scholar
Galvao Filho, Bruno Google Scholar
Santos, Luara Isabela dos Google Scholar
Sales Junior, Policarpo Ademar Google Scholar
Marques, Pedro Elias Google Scholar
Sousa Pereira, Rafaela Vaz Google Scholar
Cara, Denise Carmona Google Scholar
Bruna-Romero, Oscar Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Gazzinelli, Ricardo Tostes Google Scholar
Machado, Alexandre Vieira Google Scholar
Instituição Universidade Federal de Minas Gerais (UFMG)
Fiocruz MS
Universidade Federal de São Paulo (UNIFESP)
Univ Massachusetts
Resumo In the present study we evaluated the protection raised by immunization with recombinant influenza viruses carrying sequences coding for polypeptides corresponding to medial and carboxi-terminal moieties of Trypanosoma cruzis amastigote surface protein 2 (ASP2). Those viruses were used in sequential immunization with recombinant adenovirus (heterologous prime-boost immunization protocol) encoding the complete sequence of ASP2 (Ad-ASP2) in two mouse strains (C57BL/6 and C3H/He). the CD8 effector response elicited by this protocol was comparable to that observed in mice immunized twice with Ad-ASP2 and more robust than that observed in mice that were immunized once with Ad-ASP2. Whereas a single immunization with Ad-ASP2 sufficed to completely protect C57BL/6 mice, a higher survival rate was observed in C3H/He mice that were primed with recombinant influenza virus and boosted with Ad-ASP2 after being challenged with T. cruzi. Analyzing the phenotype of CD8+ T cells obtained from spleen of vaccinated C3H/He mice we observed that heterologous prime-boost immunization protocol elicited more CD8+ T cells specific for the immunodominant epitope as well as a higher number of CD8+ T cells producing TNF-alpha and IFN-gamma and a higher mobilization of surface marker CD107a. Taken together, our results suggest that immunodominant subpopulations of CD8+ T elicited after immunization could be directly related to degree of protection achieved by different immunization protocols using different viral vectors. Overall, these results demonstrated the usefulness of recombinant influenza viruses in immunization protocols against Chagas Disease.
Idioma Inglês
Financiador FIOCRUZ/PDTIS-Vacinas
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
Número do financiamento CNPq: 015/2008
CNPq: 064/2008
Data de publicação 2013-04-24
Publicado em Plos One. San Francisco: Public Library Science, v. 8, n. 4, 11 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, fator de impacto)
Publicador Public Library Science
Extensão 11
Fonte http://dx.doi.org/10.1371/journal.pone.0061795
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000318340400052
Endereço permanente http://repositorio.unifesp.br/handle/11600/36217

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