A heparin-like compound isolated from a marine crab rich in glucuronic acid 2-O-sulfate presents low anticoagulant activity

A heparin-like compound isolated from a marine crab rich in glucuronic acid 2-O-sulfate presents low anticoagulant activity

Autor Andrade, Giulianna P. V. Google Scholar
Lima, Marcelo Andrade de Autor UNIFESP Google Scholar
Souza Junior, Airton Araujo de Autor UNIFESP Google Scholar
Fareed, Jawed Google Scholar
Hoppensteadt, Debra A. Google Scholar
Santos, Elizeu A. Google Scholar
Chavante, Suely F. Google Scholar
Oliveira, Fernanda W. Google Scholar
Rocha, Hugo A. O. Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Instituição Univ Fed Rio Grande do Norte
Universidade Federal de São Paulo (UNIFESP)
Univ Liverpool
Inst Fed Rio Grande Norte
Loyola Med Sch
Resumo A natural heparin-like compound isolated from the crab Goniopsis cruentata was structurally characterized and its anticoagulant and hemorrhagic activities were determined. Enzymatic and nuclear magnetic resonance analysis revealed that its structure is rich in disulfated disaccharides, possessing significant amounts of 2-O-sulfated-beta-D-glucuronic acid units. Furthermore, low amounts of trisulfated disaccharide units containing 2-O-sulfated-alpha-L-iduronic acid were detected, when compared to mammalian heparin. in addition, this heparin-like structure showed negligible in vitro anticoagulant activity and low bleeding potency, facts that make it a suitable candidate for the development of structure-driven, heparin based therapeutic agents with fewer undesirable effects. (C) 2013 Elsevier B.V. All rights reserved.
Palavra-chave Heparin
Heparan sulfate
Nuclear magnetic resonance
Heparinases
Anticoagulant activity
Hemorrhagic effect
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2013-04-15
Publicado em Carbohydrate Polymers. Oxford: Elsevier B.V., v. 94, n. 1, p. 647-654, 2013.
ISSN 0144-8617 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 647-654
Fonte http://dx.doi.org/10.1016/j.carbpol.2013.01.069
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000317888500085
Endereço permanente http://repositorio.unifesp.br/handle/11600/36206

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