Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

Oleic, Linoleic and Linolenic Acids Increase ROS Production by Fibroblasts via NADPH Oxidase Activation

Autor Hatanaka, Elaine Google Scholar
Dermargos, Alexandre Google Scholar
Hirata, Aparecida Emiko Autor UNIFESP Google Scholar
Ramirez Vinolo, Marco Aurelio Google Scholar
Carpinelli, Angelo Rafael Google Scholar
Newsholme, Philip Google Scholar
Armelin, Hugo Aguirre Google Scholar
Curi, Rui Google Scholar
Instituição Cruzeiro do Sul Univ
Universidade de São Paulo (USP)
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Univ Coll Dublin
Curtin Univ
Resumo The effect of oleic, linoleic and gamma-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and gamma-linolenic acids. ROS production was dependent on the addition of beta-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and gamma-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47(phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47(phox) mRNA expression was observed using real-time PCR. the rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: gamma-linolenic > linoleic > oleic. in conclusion, oleic, linoleic and gamma-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Health Research Board of Ireland
Número do financiamento FAPESP: 08/51273-9
FAPESP: 2006/52043-1
FAPESP: 2004/12466-5
CNPq: 306004/2008-9
CNPq: 480769/2007-9
Data de publicação 2013-04-08
Publicado em Plos One. San Francisco: Public Library Science, v. 8, n. 4, 8 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, fator de impacto)
Publicador Public Library Science
Extensão 8
Fonte http://dx.doi.org/10.1371/journal.pone.0058626
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000317898000004
Endereço permanente http://repositorio.unifesp.br/handle/11600/36199

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