Diagnosis by real-time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections

Diagnosis by real-time polymerase chain reaction of pathogens and antimicrobial resistance genes in bone marrow transplant patients with bloodstream infections

Autor Menezes, Liana Carballo Autor UNIFESP Google Scholar
Rocchetti, Talita Trevizani Autor UNIFESP Google Scholar
Bauab, Karen de Castro Autor UNIFESP Google Scholar
Cappellano, Paola Autor UNIFESP Google Scholar
Quiles, Milene Goncalves Autor UNIFESP Google Scholar
Carlesse, Fabianne Autor UNIFESP Google Scholar
Oliveira, José Salvador Rodrigues de Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
GRAACC Grp Apoio Adolescente & Crianca Canc Suppo
Resumo Background: Early identification of pathogens and antimicrobial resistance in bloodstream infections (BSIs) decreases morbidity and mortality, particularly in immunocompromised patients. the aim of the present study was to compare real-time polymerase chain reaction (PCR) with commercial kits for detection of 17 pathogens from blood culture (BC) and 10 antimicrobial resistance genes.Methods: A total of 160 BCs were taken from bone marrow transplant patients and screened with Gram-specific probes by multiplex real-time PCR and 17 genus-specific sequences using TaqMan probes and blaSHV, blaTEM, blaCTX, blaKPC, blaIMP, blaSPM, blaVIM, vanA, vanB, and mecA genes by SYBR Green.Results: Twenty-three of 33 samples identified by phenotypic testing were concordantly positive by BC and real-time PCR. Pathogen identification was discordant in 13 cases. in 12 of 15 coagulase-negative staphylococci, the mecA gene was detected and four Enterococcus spp. were positive for vanA. Two blaCTX and three blaSHV genes were found by quantitative PCR. the blaKPC and metallo-beta-lactamase genes were not detected. Five fungal species were identified only by real-time PCR.Conclusions: Real-time PCR could be a valuable complementary tool in the management of BSI in bone marrow transplants patients, allowing identification of pathogens and antimicrobial resistance genes.
Palavra-chave Bone marrow transplants
Real-time polymerase chain reaction
Antibiotic resistance genes
Bloodstream infections
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Número do financiamento CNPq: 141636/2008-4
Data de publicação 2013-04-05
Publicado em Bmc Infectious Diseases. London: Biomed Central Ltd, v. 13, 7 p., 2013.
ISSN 1471-2334 (Sherpa/Romeo, fator de impacto)
Publicador Biomed Central Ltd
Extensão 7
Fonte http://dx.doi.org/10.1186/1471-2334-13-166
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000317237700001
Endereço permanente http://repositorio.unifesp.br/handle/11600/36198

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