LONG-TERM TREATMENT WITH STANDARDIZED EXTRACT of GINKGO BILOBA L. ENHANCES the CONDITIONED SUPPRESSION of LICKING in RATS BY the MODULATION of NEURONAL and GLIAL CELL FUNCTION in the DORSAL HIPPOCAMPUS and CENTRAL AMYGDALA

LONG-TERM TREATMENT WITH STANDARDIZED EXTRACT of GINKGO BILOBA L. ENHANCES the CONDITIONED SUPPRESSION of LICKING in RATS BY the MODULATION of NEURONAL and GLIAL CELL FUNCTION in the DORSAL HIPPOCAMPUS and CENTRAL AMYGDALA

Author Oliveira, D. R. Autor UNIFESP Google Scholar
Sanada, P. F. Autor UNIFESP Google Scholar
Filho, A. C. S. Autor UNIFESP Google Scholar
Conceicao, G. M. S. Autor UNIFESP Google Scholar
Cerutti, J. M. Autor UNIFESP Google Scholar
Cerutti, S. M. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Our group previously demonstrated that short-term treatment with a standardized extract of Ginkgo biloba (EGb) changed fear-conditioned memory by modulating gene expression in the hippocampus, amygdaloid complex and prefrontal cortex. Although there are few controlled studies that support the long-term use of EGb for the prevention and/or treatment of memory impairment, the chronic use of Ginkgo is common. This study evaluated the effects of chronic treatment with EGb on the conditioned emotional response, assessed by the suppression of ongoing behavior and in the modulation of gene and protein expression. Male adult Wistar rats were treated over 28 days and assigned to five groups (n = 10) as follows: positive control (4 mg kg(-1) Diazepam), negative control (12% Tween 80), EGb groups (0.5 and 1.0 g kg(-1)) and the naive group. the suppression of the licking response was calculated for each rat in six trials. Our results provide further evidence for the efficacy of EGb on memory. for the first time, we show that long-term treatment with the highest dose of EGb improves the fear memory and suggests that increased cAMP-responsive element-binding protein (CREB)-1 and glial fibrillary acidic protein (GFAP) mRNA and protein (P < 0.001) in the dorsal hippocampus and amygdaloid complex and reduced growth and plasticity-associated protein 43 (GAP-43) (P < 0.01) in the hippocampus are involved in this process. the fear memory/treatment-dependent changes observed in our study suggest that EGb might be effective for memory enhancement through its effect on the dorsal hippocampus and amygdaloid complex. (C) 2013 Published by Elsevier B.V. on behalf of IBRO.
Keywords Ginkgo biloba
conditioned suppression
dorsal hippocampus
amygdala
CREB-1
GFAP
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 02-09457-9
CAPES: 05-60330-8
Date 2013-04-03
Published in Neuroscience. Oxford: Pergamon-Elsevier B.V., v. 235, p. 70-86, 2013.
ISSN 0306-4522 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 70-86
Origin http://dx.doi.org/10.1016/j.neuroscience.2013.01.009
Access rights Closed access
Type Article
Web of Science ID WOS:000316973800008
URI http://repositorio.unifesp.br/handle/11600/36196

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