Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family

Predicting the Proteins of Angomonas deanei, Strigomonas culicis and Their Respective Endosymbionts Reveals New Aspects of the Trypanosomatidae Family

Autor Motta, Maria Cristina Machado Google Scholar
Azevedo Martins, Allan Cezar de Google Scholar
Souza, Silvana Sant'Anna de Google Scholar
Costa Catta-Preta, Carolina Moura Google Scholar
Silva, Rosane Google Scholar
Klein, Cecilia Coimbra Google Scholar
Paula de Almeida, Luiz Gonzaga Google Scholar
Cunha, Oberdan de Lima Google Scholar
Ciapina, Luciane Prioli Google Scholar
Brocchi, Marcelo Google Scholar
Colabardini, Ana Cristina Google Scholar
Lima, Bruna de Araujo Google Scholar
Machado, Carlos Renato Google Scholar
Almeida Soares, Celia Maria de Google Scholar
Probst, Christian Macagnan Google Scholar
Afonso de Menezes, Claudia Beatriz Google Scholar
Thompson, Claudia Elizabeth Google Scholar
Bartholomeu, Daniella Castanheira Google Scholar
Gradia, Daniela Fiori Google Scholar
Pavoni, Daniela Parada Google Scholar
Grisard, Edmundo C. Google Scholar
Fantinatti-Garboggini, Fabiana Google Scholar
Marchini, Fabricio Klerynton Google Scholar
Rodrigues-Luiz, Gabriela Flaia Google Scholar
Wagner, Glauber Google Scholar
Goldman, Gustavo Henrique Google Scholar
Rangel Fietto, Juliana Lopes Google Scholar
Elias, Maria Carolina Google Scholar
Goldman, Maria Helena S. Google Scholar
Sagot, Marie-France Google Scholar
Pereira, Maristela Google Scholar
Stoco, Patricia H. Google Scholar
Mendonca-Neto, Rondon Pessoa de Google Scholar
Ribeiro Teixeira, Santuza Maria Google Scholar
Ferreira Maciel, Talles Eduardo Google Scholar
Oliveira Mendes, Tiago Antonio de Google Scholar
Uermenyi, Turan P. Google Scholar
Souza, Wanderley de Google Scholar
Schenkman, Sergio Autor UNIFESP Google Scholar
Ribeiro de Vasconcelos, Ana Tereza Google Scholar
Instituição Universidade Federal do Rio de Janeiro (UFRJ)
Lab Bioinformat
INRIA Grenoble Rhone Alpes
Univ Lyon 1
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Lab Nacl Ciencia & Tecnol Bioetano
Universidade Federal de Minas Gerais (UFMG)
Universidade Federal de Goiás (UFG)
Fundacao Oswaldo Cruz
Universidade Federal de Santa Catarina (UFSC)
Universidade Federal de Viçosa (UFV)
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Resumo Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomatidae family, thus classified as monoxenics. in an effort to better understand such symbiotic association, we used DNA pyrosequencing and a reference-guided assembly to generate reads that predicted 16,960 and 12,162 open reading frames (ORFs) in two symbiont-bearing trypanosomatids, Angomonas deanei (previously named as Crithidia deanei) and Strigomonas culicis (first known as Blastocrithidia culicis), respectively. Identification of each ORF was based primarily on TriTrypDB using tblastn, and each ORF was confirmed by employing getorf from EMBOSS and Newbler 2.6 when necessary. the monoxenic organisms revealed conserved housekeeping functions when compared to other trypanosomatids, especially compared with Leishmania major. However, major differences were found in ORFs corresponding to the cytoskeleton, the kinetoplast, and the paraflagellar structure. the monoxenic organisms also contain a large number of genes for cytosolic calpain-like and surface gp63 metalloproteases and a reduced number of compartmentalized cysteine proteases in comparison to other TriTryp organisms, reflecting adaptations to the presence of the symbiont. the assembled bacterial endosymbiont sequences exhibit a high A+T content with a total of 787 and 769 ORFs for the Angomonas deanei and Strigomonas culicis endosymbionts, respectively, and indicate that these organisms hold a common ancestor related to the Alcaligenaceae family. Importantly, both symbionts contain enzymes that complement essential host cell biosynthetic pathways, such as those for amino acid, lipid and purine/pyrimidine metabolism. These findings increase our understanding of the intricate symbiotic relationship between the bacterium and the trypanosomatid host and provide clues to better understand eukaryotic cell evolution.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data 2013-04-03
Publicado em Plos One. San Francisco: Public Library Science, v. 8, n. 4, 20 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, fator de impacto)
Editor Public Library Science
Extensão 20
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000318840100054

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