The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1 beta Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp bolletii

The Detection and Sequencing of a Broad-Host-Range Conjugative IncP-1 beta Plasmid in an Epidemic Strain of Mycobacterium abscessus subsp bolletii

Autor Leao, Sylvia Cardoso Autor UNIFESP Google Scholar
Matsumoto, Cristianne Kayoko Autor UNIFESP Google Scholar
Carneiro, Adriana Google Scholar
Ramos, Rommel Thiago Google Scholar
Nogueira, Christiane Lourenco Autor UNIFESP Google Scholar
Lima Junior, James Daltro Autor UNIFESP Google Scholar
Lima, Karla Valeria Google Scholar
Lopes, Maria Luiza Google Scholar
Schneider, Horacio Google Scholar
Azevedo, Vasco Ariston Google Scholar
Silva, Artur da Costa da Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Inst Evandro Chagas
Universidade Federal de Minas Gerais (UFMG)
Resumo Background: An extended outbreak of mycobacterial surgical infections occurred in Brazil during 2004-2008. Most infections were caused by a single strain of Mycobacterium abscessus subsp. bolletii, which was characterized by a specific rpoB sequevar and two highly similar pulsed-field gel electrophoresis (PFGE) patterns differentiated by the presence of a similar to 50 kb band. the nature of this band was investigated.Methodology/Principal Findings: Genomic sequencing of the prototype outbreak isolate INCQS 00594 using the SOLiD platform demonstrated the presence of a 56,264-bp circular plasmid, designated pMAB01. Identity matrices, genetic distances and phylogeny analyses indicated that pMAB01 belongs to the broad-host-range plasmid subgroup IncP-1 beta and is highly related to BRA100, pJP4, pAKD33 and pB10. the presence of pMAB01-derived sequences in 41 M. abscessus subsp. bolletii isolates was evaluated using PCR, PFGE and Southern blot hybridization. Sixteen of the 41 isolates showed the presence of the plasmid. the plasmid was visualized as a similar to 50-kb band using PFGE and Southern blot hybridization in 12 isolates. the remaining 25 isolates did not exhibit any evidence of this plasmid. the plasmid was successfully transferred to Escherichia coli by conjugation and transformation. Lateral transfer of pMAB01 to the high efficient plasmid transformation strain Mycobacterium smegmatis mc(2)155 could not be demonstrated.Conclusions/Significance: the occurrence of a broad-host-range IncP-1 beta plasmid in mycobacteria is reported for the first time. Thus, genetic exchange could result in the emergence of specific strains that might be better adapted to cause human disease.
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Fundao de Amparo a Pesquisa do Estado de São Paulo
Número do financiamento FAPESP: FAPESP - 06/01533-9
Fundao de Amparo a Pesquisa do Estado de São Paulo: FAPESP - 8/01451-8
Data de publicação 2013-04-02
Publicado em Plos One. San Francisco: Public Library Science, v. 8, n. 4, 14 p., 2013.
ISSN 1932-6203 (Sherpa/Romeo, fator de impacto)
Publicador Public Library Science
Extensão 14
Fonte http://dx.doi.org/10.1371/journal.pone.0060746
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000317717300145
Endereço permanente http://repositorio.unifesp.br/handle/11600/36193

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