Plasmatic higher levels of homocysteine in Non-alcoholic fatty liver disease (NAFLD)

Plasmatic higher levels of homocysteine in Non-alcoholic fatty liver disease (NAFLD)

Autor Carvalho, Sylene Coutinho Rampche de Google Scholar
Muniz, Maria Tereza Cartaxo Google Scholar
Siqueira, Maria Deozete Vieira Google Scholar
Siqueira, Erika Rabelo Forte Google Scholar
Gomes, Adriana Vieira Google Scholar
Silva, Karina Alves Google Scholar
Bezerra, Lais Carvalho Luma Google Scholar
D'Almeida, Vania Autor UNIFESP Google Scholar
Oliveira, Claudia Pinto Marques Souza de Google Scholar
Pereira, Leila Maria M. Beltrao Google Scholar
Instituição Univ Pernambuco
Liver Inst Pernambuco
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Inst Figado Pernambuco
Resumo Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease, which includes a spectrum of hepatic pathology such as simple steatosis, steatohepatitis, fibrosis and cirrhosis. the increased serum levels of homocysteine (Hcy) may be associated with hepatic fat accumulation. Genetic mutations in the folate route may only mildly impair Hcy metabolism. the aim of this study was to investigate the relation between liver steatosis with plasma homocysteine level and MTHFR C677T and A1298C polymorphisms in Brazilian patients with NAFLD.Methods: Thirty-five patients diagnosed with NAFLD by liver biopsy and forty-five healthy controls neither age nor sex matched were genotyped for C677T and A1298C MTHFR polymorphisms using PCR-RFLP and PCR-ASA, respectively, and Hcy was determined by HPLC. All patients were negative for markers of Wilson's, hemochromatosis and autoimmune diseases. Their daily alcohol intake was less than 100 g/week. A set of metabolic and serum lipid markers were also measured at the time of liver biopsies.Results: the plasma Hcy level was higher in NAFLD patients compared to the control group (p = 0.0341). No statistical difference for genotypes 677C/T (p = 0.110) and 1298A/C (p = 0.343) in patients with NAFLD and control subjects was observed. the genotypes distribution was in Hardy-Weinberg equilibrium (677C/T p = 0.694 and 1298 A/C p = 0.188). the group of patients and controls showed a statistically significant difference (p < 0.001) for BMI and HOMA_IR, similarly to HDL cholesterol levels (p < 0,006), AST, ALT, gamma GT, AP and triglycerides levels (p < 0.001). A negative correlation was observed between levels of vitamin B12 and Hcy concentration (p = 0.005).Conclusion: Our results indicate that plasma Hcy was higher in NAFLD than controls. the MTHFR C677T and A1298C polymorphisms did not differ significantly between groups, despite the 677TT homozygous frequency was higher in patients (17.14%) than in controls (677TT = 4.44%) (p > 0.05). the suggested genetic susceptibility to the MTHFR C677T and A1298C should be confirmed in large population based studies.
Assunto Fatty liver
Non-alcoholic steatohepatitis
Methylenetetrahydrofolate reductase (MTHFR)
Oxidative stress
Polymorphisms
Idioma Inglês
Data 2013-04-02
Publicado em Nutrition Journal. London: Biomed Central Ltd, v. 12, 5 p., 2013.
ISSN 1475-2891 (Sherpa/Romeo, fator de impacto)
Editor Biomed Central Ltd
Extensão 5
Fonte http://dx.doi.org/10.1186/1475-2891-12-37
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000317666000001
URI http://repositorio.unifesp.br/handle/11600/36192

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