Study of Tryptophan Lifetime Fluorescence Following Low-Density Lipoprotein Modification

Study of Tryptophan Lifetime Fluorescence Following Low-Density Lipoprotein Modification

Author Sicchieri, Leticia Bonfante Google Scholar
Monteiro, Andrea Moreira Google Scholar
Samad, Ricardo Elgul Google Scholar
Ito, Amando Siuiti Google Scholar
Figueiredo Neto, Antonio Martins Google Scholar
Vieira, Nilson Dias Google Scholar
Gidlund, Magnus Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Institution Comissao Nacl Energia Nucl
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract In this paper we report the effects of the irradiation of low-density lipoprotein (LDL) by ultra-short laser pulses to obtain in vitro alterations mimicking proatherogenic modifications occurring in vivo in LDL. the modifications by metallic ions (copper and iron) and ultra-short laser pulses were studied by fluorescence steady state and time-resolved lifetime measurements. the results demonstrate that the modifications caused by ultra-short laser pulses and by iron affect the tryptophan residues of apolipoprotein B-100 (Apo-B), slightly decreasing fluorescent lifetimes, with almost no modifications in pre-exponential factors, indicating preservation of structural properties around the fluorophore. On the other hand, oxidation by copper strongly affects the Apo-B protein associated with LDL. We describe a fast, inexpensive, and nondestructive fluorescence-based method that is readily accessible to provide the LDL particle characterization.
Keywords Low-density lipoprotein (LDL)
Time-resolved fluorescence
Tryptophan
Ultra-short pulsed laser
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Instituto Nacional de Ciencia e Tecnologia de Fluidos Complexos
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-04-01
Published in Applied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 67, n. 4, p. 379-384, 2013.
ISSN 0003-7028 (Sherpa/Romeo, impact factor)
Publisher Soc Applied Spectroscopy
Extent 379-384
Origin http://dx.doi.org/10.1366/12-06780
Access rights Closed access
Type Article
Web of Science ID WOS:000317168100007
URI http://repositorio.unifesp.br/handle/11600/36190

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