A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar rats

A high-fat diet increases interleukin-3 and granulocyte colony-stimulating factor production by bone marrow cells and triggers bone marrow hyperplasia and neutrophilia in wistar rats

Autor Carmo, Luciana Simao do Google Scholar
Rogero, Marcelo Macedo Google Scholar
Paredes-Gamero, Edgar Julian Autor UNIFESP Google Scholar
Nogueira-Pedro, Amanda Autor UNIFESP Google Scholar
Xavier, Jose Guilherme Google Scholar
Cortez, Mayara Google Scholar
Borges, Maria Carolina Google Scholar
Fujii, Tatiane Mieko Google Scholar
Borelli, Primavera Google Scholar
Fock, Ricardo Ambrosio Google Scholar
Instituição Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Univ Estadual Paulista
Resumo It is well established that the excessive consumption of a high-fat diet (HFD) results in overweight, obesity and an increase in leptin concentrations, which triggers a chronic inflammatory condition that is associated with a high white blood cell count. Two-month-old male Wistar rats were fed a control (CON) diet or an HFD for 12 weeks. After this period, hemogram, myelogram and biochemical parameters were evaluated along with the cell cycle and the percentage of CD34(+) cells in the bone marrow as well as cell proliferation and differentiation assays and the production of stem cell factor, interleukin 3 (IL-3), granulocyte colony-stimulating factor (G-CSF) and granulocyte macrophage colony-stimulating factor (GM-CSF). the HFD animals exhibited leukocytosis and neutrophilia with increased C-reactive protein, leptin, cholesterol and triglyceride concentrations. in the HFD group, the bone marrow revealed myeloid hyperplasia, especially of the granulocytic compartment with a higher percentage of CD34(+) cells and a higher percentage of cells in the G2/S/M cell cycle phases. in addition, the HFD bone marrow cells had a higher capacity to proliferate and differentiate into granulocytic cells in an in vitro system and a higher capacity to produce IL-3 and G-CSF. These data led us to infer that the HFD induces leukocytosis and neutrophilia suggesting alterations in hematopoiesis system modulation.
Palavra-chave high-fat diet
leukocytosis
neutrophilia
IL-3
G-CSF
CD34 cells
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Data de publicação 2013-04-01
Publicado em Experimental Biology and Medicine. London: Royal Soc Medicine Press Ltd, v. 238, n. 4, p. 375-384, 2013.
ISSN 1535-3702 (Sherpa/Romeo, fator de impacto)
Publicador Royal Soc Medicine Press Ltd
Extensão 375-384
Fonte http://dx.doi.org/10.1177/1535370213477976
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000321757900006
Endereço permanente http://repositorio.unifesp.br/handle/11600/36189

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