Shiga toxin-producing Escherichia coli in drinking water supplies of north Parana State, Brazil

Shiga toxin-producing Escherichia coli in drinking water supplies of north Parana State, Brazil

Autor Lascowski, K. M. S. Autor UNIFESP Google Scholar
Guth, B. E. C. Autor UNIFESP Google Scholar
Martins, F. H. Google Scholar
Rocha, S. P. D. Google Scholar
Irino, K. Google Scholar
Pelayo, J. S. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Londrina (UEL)
Adolfo Lutz Inst
Resumo Aim To determine the occurrence and characteristics of Shiga toxin-producing Escherichia coli (STEC) in drinking water supplies treated and untreated. Methods and Results Drinking water samples (n=1850) were collected from 41 municipalities in the north of Parana State between February 2005 and January 2006. Escherichia coli isolates (n=300) were recovered from water and investigated for the presence of virulence markers related to STEC by PCR. STEC isolates recovered were then characterized for both phenotypic and genotypic traits. A total of 12 isolates (11 from untreated water and one from treated water) were positive for stx, including five positive for both stx1 and stx2, two positive for stx1 and five positive for stx2. None of the STEC isolates contained eae, but other virulence genes were observed such as ehxA (100%), saa (100%), lpfAO113 (75%), iha (42%), subAB (25%) and cdtV (8%). Multidrug resistance was identified in 25% of the STEC isolates. the 12 STEC isolates belonged to seven distinct serotypes and pulsed-field gel electrophoresis typing revealed the presence of two clusters and two clones in this region. Conclusion Drinking water, especially from untreated water supplies, can be source of STEC strains potentially pathogenic for humans. Significance and Impact of the Study the investigation of the drinking water supplies for pathogenic E.coli, as STEC, may be useful to prevent waterborne outbreaks.
Palavra-chave antibiotics
resistance
drinking water
pulsed-field gel electrophoresis
Shiga toxin-producing Escherichia coli
virulence markers
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data de publicação 2013-04-01
Publicado em Journal of Applied Microbiology. Hoboken: Wiley-Blackwell, v. 114, n. 4, p. 1230-1239, 2013.
ISSN 1364-5072 (Sherpa/Romeo, fator de impacto)
Publicador Wiley-Blackwell
Extensão 1230-1239
Fonte http://dx.doi.org/10.1111/jam.12113
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000316572200032
Endereço permanente http://repositorio.unifesp.br/handle/11600/36184

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