Microbiota Evaluation of Patients With a Boston Type I Keratoprosthesis Treated With Topical 0.5% Moxifloxacin and 5% Povidone-Iodine

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dc.contributor.author Magalhaes, Fernanda Pedreira [UNIFESP]
dc.contributor.author Nascimento, Heloisa Moraes do [UNIFESP]
dc.contributor.author Ecker, David J.
dc.contributor.author Sannes-Lowery, Kristin A.
dc.contributor.author Sampath, Rangarajan
dc.contributor.author Rosenblatt, Mark I.
dc.contributor.author Sousa, Luciene Barbosa de [UNIFESP]
dc.contributor.author Oliveira, Lauro Augusto de [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:32Z
dc.date.available 2016-01-24T14:31:32Z
dc.date.issued 2013-04-01
dc.identifier http://dx.doi.org/10.1097/ICO.0b013e31824a8b9b
dc.identifier.citation Cornea. Philadelphia: Lippincott Williams & Wilkins, v. 32, n. 4, p. 407-411, 2013.
dc.identifier.issn 0277-3740
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36181
dc.description.abstract Purpose: To evaluate the efficacy of a prophylactic regimen of daily topical 0.5% moxifloxacin and 5% povidone-iodine (PI) in patients with Boston type I keratoprosthesis (KPro) and to assess the applicability of a novel molecular diagnostic technique to analyze the ocular surface microbiota in these patients.Methods: Ten patients had their inferior conjunctival fornix sampled for standard culture methods before the addition of topical 5% PI to the prophylactic regimen and were considered the control group (group 1). the inferior conjunctival fornix and the KPro-donor cornea interface of 10 patients treated with the mentioned prophylactic regimen were sampled and analyzed by standard culture methods and using a polymerase chain reaction/electrospray ionization mass spectrometry assay (group 2).Results: Samples from the inferior conjunctival fornix were positive for coagulase-negative staphylococcus in 3 patients and for Aerobasidium pullulans in 1 patient in group 1. the inferior conjunctival fornix and the KPro-donor cornea interface scrapings were positive for coagulase-negative staphylococcus in 2 patients and 1 patient, respectively, in group 2. No bacteria and fungi growth were detected in any patient from group 2 with the molecular diagnostic approach. None of the patients with culture-positive results developed keratitis or endophthalmitis during the study.Conclusions: Topical 0.5% moxifloxacin associated with topical 5% PI is an effective prophylactic regimen in patients with Boston type I KPro. the molecular diagnostic approach using serial polymerase chain reaction and mass spectrometry was comparable with standard microbiologic techniques as a surveillance tool in these patients. en
dc.description.sponsorship Ibis Biosciences
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 407-411
dc.language.iso eng
dc.publisher Lippincott Williams & Wilkins
dc.relation.ispartof Cornea
dc.rights Acesso restrito
dc.subject keratoprosthesis en
dc.subject polymerase chain reaction en
dc.subject keratitis en
dc.title Microbiota Evaluation of Patients With a Boston Type I Keratoprosthesis Treated With Topical 0.5% Moxifloxacin and 5% Povidone-Iodine en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Ibis Biosci Inc
dc.contributor.institution Weill Cornell Med Coll
dc.description.affiliation Universidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, Brazil
dc.description.affiliation Ibis Biosci Inc, Carlsbad, CA USA
dc.description.affiliation Weill Cornell Med Coll, Dept Ophthalmol, New York, NY USA
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Ophthalmol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1097/ICO.0b013e31824a8b9b
dc.description.source Web of Science
dc.identifier.wos WOS:000315999500016



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