Microbiota Evaluation of Patients With a Boston Type I Keratoprosthesis Treated With Topical 0.5% Moxifloxacin and 5% Povidone-Iodine

Microbiota Evaluation of Patients With a Boston Type I Keratoprosthesis Treated With Topical 0.5% Moxifloxacin and 5% Povidone-Iodine

Autor Magalhaes, Fernanda Pedreira Autor UNIFESP Google Scholar
Nascimento, Heloisa Moraes do Autor UNIFESP Google Scholar
Ecker, David J. Google Scholar
Sannes-Lowery, Kristin A. Google Scholar
Sampath, Rangarajan Google Scholar
Rosenblatt, Mark I. Google Scholar
Sousa, Luciene Barbosa de Autor UNIFESP Google Scholar
Oliveira, Lauro Augusto de Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Ibis Biosci Inc
Weill Cornell Med Coll
Resumo Purpose: To evaluate the efficacy of a prophylactic regimen of daily topical 0.5% moxifloxacin and 5% povidone-iodine (PI) in patients with Boston type I keratoprosthesis (KPro) and to assess the applicability of a novel molecular diagnostic technique to analyze the ocular surface microbiota in these patients.Methods: Ten patients had their inferior conjunctival fornix sampled for standard culture methods before the addition of topical 5% PI to the prophylactic regimen and were considered the control group (group 1). the inferior conjunctival fornix and the KPro-donor cornea interface of 10 patients treated with the mentioned prophylactic regimen were sampled and analyzed by standard culture methods and using a polymerase chain reaction/electrospray ionization mass spectrometry assay (group 2).Results: Samples from the inferior conjunctival fornix were positive for coagulase-negative staphylococcus in 3 patients and for Aerobasidium pullulans in 1 patient in group 1. the inferior conjunctival fornix and the KPro-donor cornea interface scrapings were positive for coagulase-negative staphylococcus in 2 patients and 1 patient, respectively, in group 2. No bacteria and fungi growth were detected in any patient from group 2 with the molecular diagnostic approach. None of the patients with culture-positive results developed keratitis or endophthalmitis during the study.Conclusions: Topical 0.5% moxifloxacin associated with topical 5% PI is an effective prophylactic regimen in patients with Boston type I KPro. the molecular diagnostic approach using serial polymerase chain reaction and mass spectrometry was comparable with standard microbiologic techniques as a surveillance tool in these patients.
Palavra-chave keratoprosthesis
polymerase chain reaction
keratitis
Idioma Inglês
Financiador Ibis Biosciences
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2013-04-01
Publicado em Cornea. Philadelphia: Lippincott Williams & Wilkins, v. 32, n. 4, p. 407-411, 2013.
ISSN 0277-3740 (Sherpa/Romeo, fator de impacto)
Publicador Lippincott Williams & Wilkins
Extensão 407-411
Fonte http://dx.doi.org/10.1097/ICO.0b013e31824a8b9b
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000315999500016
Endereço permanente http://repositorio.unifesp.br/handle/11600/36181

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