Immunohistochemistry and ultrastructural characteristics of nerve endings in the oral mucosa of rat

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dc.contributor.author Watanabe, Ii-Sei
dc.contributor.author Dias, Fernando Jose
dc.contributor.author Mardegan Issa, Joao Paulo
dc.contributor.author Santos Haemmerle, Carlos Alexandre dos
dc.contributor.author Cury, Diego Pulzatto
dc.contributor.author Takada, Silvia Honda
dc.contributor.author Kronka Sosthenes, Marcia Consentino
dc.contributor.author Silva, Marcelo Cavenaghi Pereira da [UNIFESP]
dc.contributor.author Campos, Leila M. G.
dc.contributor.author Nogueira, Maria Ines
dc.contributor.author Iyomasa, Mamie Mizusaki
dc.date.accessioned 2016-01-24T14:31:32Z
dc.date.available 2016-01-24T14:31:32Z
dc.date.issued 2013-04-01
dc.identifier http://dx.doi.org/10.1093/jmicro/dfs068
dc.identifier.citation Journal of Electron Microscopy. Oxford: Oxford Univ Press, v. 62, n. 2, p. 259-270, 2013.
dc.identifier.issn 0022-0744
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36179
dc.description.abstract The sensory nerve endings of the rat tongue, cheek and palate were studied using immunohistochemical staining and transmission electron microscopy analysis. the specimens were fixed in modified Karnovsky solution and embedded in Spurr resin. Substance P, calcitonin gene-related peptide (CGRP)- and protein gene product 9.5 (PGP b9.5)-containing nerve fibers in the rat tongue, cheek and palate were examined by electronic microscopical analysis and immunohistochemical localization. These fibers run very close to the basal lamina of the epithelium and extend into the filliform and fungiform papillae. Numerous plexiform fibers immunoreactive for substance P, CGRP and PGP 9.5 were found in the connective tissue of mucosa. Electron microscopic observations showed clearly immunostained nerve fibers, which are located very close to the basal lamina of epithelial cells. Some electron-dense granules may be observed in the axoplasms of both substance P and CGRP immunoreactive fibers. Several lamellar corpuscles into the subepithelial connective tissue papillae, Merkel corpuscles and numerous thin unmyelinated and myelinated axons were observed. the terminal axons revealed numerous mitochondria, neurofilaments, microtubules and clear vesicles in the base of axoplasmic protrusions. the lamellar cells showed caveolae and interlamelar spaces filled by amorphous substance. Between the lamellar cells and axoplasmic membrane, and in the adjacent lamellae region, desmosome-type junctions were observed. the quantitative and morphometric analysis showed nerve endings with an average area of 4.83 +/- 3.4 mu m(2) and 19.4 internal mitochondria in this site and the organized corpuscles with an average area of 79.24 +/- 27.24 mu m(2) and 24.23 internal mitochondria in this place. All the structures observed are involved in the transmission of pain and mechanoreceptors stimulus of these oral mucosae. en
dc.description.sponsorship Japan Society for the Promotion of Science
dc.description.sponsorship São Paulo State Foundation
dc.description.sponsorship Japan International Cooperation Agency
dc.format.extent 259-270
dc.language.iso eng
dc.publisher Oxford Univ Press
dc.relation.ispartof Journal of Electron Microscopy
dc.rights Acesso restrito
dc.subject Lamellated corpuscles en
dc.subject nerve endings en
dc.subject immunohistochemistry en
dc.subject mitochondria en
dc.subject TEM en
dc.subject rat en
dc.title Immunohistochemistry and ultrastructural characteristics of nerve endings in the oral mucosa of rat en
dc.type Artigo
dc.rights.license http://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Fed Univ Para
dc.description.affiliation Univ São Paulo, Inst Biomed Sci, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Fac Dent Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Dept Anat, São Paulo, Brazil
dc.description.affiliation Fed Univ Para, Univ Hosp Joao de Barros Barreto, BR-66059 Belem, Para, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Anat, São Paulo, Brazil
dc.description.sponsorshipID Japan Society for the Promotion of Science: JSPS/EP/78191
dc.description.sponsorshipID São Paulo State Foundation: FAPESP N. 97/09943-0
dc.description.sponsorshipID Japan International Cooperation Agency: JICA N. 8205491
dc.identifier.doi 10.1093/jmicro/dfs068
dc.description.source Web of Science
dc.identifier.wos WOS:000317506600003



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