Immunohistochemistry and ultrastructural characteristics of nerve endings in the oral mucosa of rat

Immunohistochemistry and ultrastructural characteristics of nerve endings in the oral mucosa of rat

Author Watanabe, Ii-Sei Google Scholar
Dias, Fernando Jose Google Scholar
Mardegan Issa, Joao Paulo Google Scholar
Santos Haemmerle, Carlos Alexandre dos Google Scholar
Cury, Diego Pulzatto Google Scholar
Takada, Silvia Honda Google Scholar
Kronka Sosthenes, Marcia Consentino Google Scholar
Silva, Marcelo Cavenaghi Pereira da Autor UNIFESP Google Scholar
Campos, Leila M. G. Google Scholar
Nogueira, Maria Ines Google Scholar
Iyomasa, Mamie Mizusaki Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Abstract The sensory nerve endings of the rat tongue, cheek and palate were studied using immunohistochemical staining and transmission electron microscopy analysis. the specimens were fixed in modified Karnovsky solution and embedded in Spurr resin. Substance P, calcitonin gene-related peptide (CGRP)- and protein gene product 9.5 (PGP b9.5)-containing nerve fibers in the rat tongue, cheek and palate were examined by electronic microscopical analysis and immunohistochemical localization. These fibers run very close to the basal lamina of the epithelium and extend into the filliform and fungiform papillae. Numerous plexiform fibers immunoreactive for substance P, CGRP and PGP 9.5 were found in the connective tissue of mucosa. Electron microscopic observations showed clearly immunostained nerve fibers, which are located very close to the basal lamina of epithelial cells. Some electron-dense granules may be observed in the axoplasms of both substance P and CGRP immunoreactive fibers. Several lamellar corpuscles into the subepithelial connective tissue papillae, Merkel corpuscles and numerous thin unmyelinated and myelinated axons were observed. the terminal axons revealed numerous mitochondria, neurofilaments, microtubules and clear vesicles in the base of axoplasmic protrusions. the lamellar cells showed caveolae and interlamelar spaces filled by amorphous substance. Between the lamellar cells and axoplasmic membrane, and in the adjacent lamellae region, desmosome-type junctions were observed. the quantitative and morphometric analysis showed nerve endings with an average area of 4.83 +/- 3.4 mu m(2) and 19.4 internal mitochondria in this site and the organized corpuscles with an average area of 79.24 +/- 27.24 mu m(2) and 24.23 internal mitochondria in this place. All the structures observed are involved in the transmission of pain and mechanoreceptors stimulus of these oral mucosae.
Keywords Lamellated corpuscles
nerve endings
immunohistochemistry
mitochondria
TEM
rat
Language English
Sponsor Japan Society for the Promotion of Science
São Paulo State Foundation
Japan International Cooperation Agency
Grant number Japan Society for the Promotion of Science: JSPS/EP/78191
São Paulo State Foundation: FAPESP N. 97/09943-0
Japan International Cooperation Agency: JICA N. 8205491
Date 2013-04-01
Published in Journal of Electron Microscopy. Oxford: Oxford Univ Press, v. 62, n. 2, p. 259-270, 2013.
ISSN 0022-0744 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 259-270
Origin http://dx.doi.org/10.1093/jmicro/dfs068
Access rights Closed access
Type Article
Web of Science ID WOS:000317506600003
URI http://repositorio.unifesp.br/handle/11600/36179

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