Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern

Show simple item record

dc.contributor.author Dellavance, Alessandra
dc.contributor.author Alvarenga, Rossana Rassi [UNIFESP]
dc.contributor.author Rodrigues, Silvia Helena [UNIFESP]
dc.contributor.author Barbosa, Silvia Helena
dc.contributor.author Pinto Camilo, Amandia Cristina
dc.contributor.author Okamoto Shiguedomi, Herika Santiago
dc.contributor.author Rodrigues, Silvia Sanchez
dc.contributor.author Silva, Cristiane Gallindo
dc.contributor.author Coelho Andrade, Luis Eduardo [UNIFESP]
dc.date.accessioned 2016-01-24T14:31:31Z
dc.date.available 2016-01-24T14:31:31Z
dc.date.issued 2013-04-01
dc.identifier http://dx.doi.org/10.1016/j.jim.2013.01.006
dc.identifier.citation Journal of Immunological Methods. Amsterdam: Elsevier B.V., v. 390, n. 1-2, p. 35-40, 2013.
dc.identifier.issn 0022-1759
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/36161
dc.description.abstract Objectives: To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro.Methods: A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro pattern and 64 samples presenting non-SS-A/Ro nuclear fine speckled pattern at the ANA-HEp-2 routine were screened for SS-A/Ro reactivity. Conversely, 48 samples with known reactivity to 60 kDa SS-A/Ro, 13 samples with exclusive reactivity to 52 kDa SS-A/Ro, and 48 SS-A/Ro-negative samples were analyzed for the ANA-HEp-2 pattern.Results: Eighty-five of the 86 samples (98.8%) presenting the SS-A/Ro pattern and 15 of the 64 (23.4%) samples with non-SS-A/Ro fine speckled pattern reacted with 60 kDa SS-A/Ro. Conversely, 44 (91.6%) of 48 samples with known reactivity to 60 kDa SS-A/Ro presented the SS-A/Ro pattern and four (8.4%) presented non-SS-A/Ro fine speckled pattern. None of the 48 anti-SS-A/Ro-negative samples and none of anti-52 kDa SS-A/Ro-positive samples yielded the SS-A/Ro pattern. This immunofluorescence pattern was observed in different commercial HEp-2 cell slides and in homemade HEp-2 cell slides.Conclusions: the SS-A/Ro pattern belongs to the group of immunofluorescence patterns that hold strong association with the respective autoantibody specificities, such as those associated with CENP-F and NuMA-1. the identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. (C) 2013 Elsevier B.V. All rights reserved. en
dc.format.extent 35-40
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Journal of Immunological Methods
dc.rights Acesso restrito
dc.subject Antinuclear antibodies en
dc.subject Autoantibodies en
dc.subject SS-A/Ro en
dc.subject Systemic lupus erythematosus en
dc.subject Sjogren's syndrome en
dc.title Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Fleury Med & Hlth Labs
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Fleury Med & Hlth Labs, Div Res & Dev, São Paulo, Brazil
dc.description.affiliation Universidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, Brazil
dc.description.affiliation Fleury Med & Hlth Labs, Div Immunol, São Paulo, Brazil
dc.description.affiliation Fleury Med & Hlth Labs, Reagents Prod Div, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, UNIFESP, Div Rheumatol, BR-04023900 São Paulo, Brazil
dc.identifier.doi 10.1016/j.jim.2013.01.006
dc.description.source Web of Science
dc.identifier.wos WOS:000317380500005



File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search


Browse

Statistics

My Account