Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern

Autoantibodies to 60 kDa SS-A/Ro yield a specific nuclear myriad discrete fine speckled immunofluorescence pattern

Autor Dellavance, Alessandra Google Scholar
Alvarenga, Rossana Rassi Autor UNIFESP Google Scholar
Rodrigues, Silvia Helena Autor UNIFESP Google Scholar
Barbosa, Silvia Helena Google Scholar
Pinto Camilo, Amandia Cristina Google Scholar
Okamoto Shiguedomi, Herika Santiago Google Scholar
Rodrigues, Silvia Sanchez Google Scholar
Silva, Cristiane Gallindo Google Scholar
Coelho Andrade, Luis Eduardo Autor UNIFESP Google Scholar
Instituição Fleury Med & Hlth Labs
Universidade Federal de São Paulo (UNIFESP)
Resumo Objectives: To report on a novel immunofluorescence pattern specifically associated with antibodies to SS-A/Ro.Methods: A novel immunofluorescence pattern, herein designated SS-A/Ro pattern, was characterized as myriad discrete fine speckles throughout the nucleus. Eighty-six sequential samples presenting the SS-A/Ro pattern and 64 samples presenting non-SS-A/Ro nuclear fine speckled pattern at the ANA-HEp-2 routine were screened for SS-A/Ro reactivity. Conversely, 48 samples with known reactivity to 60 kDa SS-A/Ro, 13 samples with exclusive reactivity to 52 kDa SS-A/Ro, and 48 SS-A/Ro-negative samples were analyzed for the ANA-HEp-2 pattern.Results: Eighty-five of the 86 samples (98.8%) presenting the SS-A/Ro pattern and 15 of the 64 (23.4%) samples with non-SS-A/Ro fine speckled pattern reacted with 60 kDa SS-A/Ro. Conversely, 44 (91.6%) of 48 samples with known reactivity to 60 kDa SS-A/Ro presented the SS-A/Ro pattern and four (8.4%) presented non-SS-A/Ro fine speckled pattern. None of the 48 anti-SS-A/Ro-negative samples and none of anti-52 kDa SS-A/Ro-positive samples yielded the SS-A/Ro pattern. This immunofluorescence pattern was observed in different commercial HEp-2 cell slides and in homemade HEp-2 cell slides.Conclusions: the SS-A/Ro pattern belongs to the group of immunofluorescence patterns that hold strong association with the respective autoantibody specificities, such as those associated with CENP-F and NuMA-1. the identification of the SS-A/Ro pattern at the ANA-HEp-2 screening routine shall lead to specific tests for the identification of anti-SS-A/Ro antibodies. (C) 2013 Elsevier B.V. All rights reserved.
Palavra-chave Antinuclear antibodies
Autoantibodies
SS-A/Ro
Systemic lupus erythematosus
Sjogren's syndrome
Idioma Inglês
Data de publicação 2013-04-01
Publicado em Journal of Immunological Methods. Amsterdam: Elsevier B.V., v. 390, n. 1-2, p. 35-40, 2013.
ISSN 0022-1759 (Sherpa/Romeo, fator de impacto)
Publicador Elsevier B.V.
Extensão 35-40
Fonte http://dx.doi.org/10.1016/j.jim.2013.01.006
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000317380500005
Endereço permanente http://repositorio.unifesp.br/handle/11600/36161

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta