Characterization of cell-free circulating DNA in plasma in patients with prostate cancer

Characterization of cell-free circulating DNA in plasma in patients with prostate cancer

Author Delgado, Pamela Oliveira Google Scholar
Alves, Beatriz Costa A. Google Scholar
Gehrke, Flavia de Sousa Google Scholar
Kuniyoshi, Renata Kelly Google Scholar
Wroclavski, Marcelo Langer Google Scholar
Del Giglio, Auro Google Scholar
Affonso Fonseca, Fernando Luiz Autor UNIFESP Google Scholar
Institution FMABC
Universidade Federal de São Paulo (UNIFESP)
Abstract Cell-free circulating DNA in plasma and serum may serve as a biomarker for malignant tumor detection and follow up in patients with a variety of solid tumors including prostate cancer. in healthy patients, DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe. Cell-free circulating DNA levels were measured by a quantitative real-time PCR method with a set of primers targeted to amplify the consensus ALU apoptotic versus necrotic origin. Prostate cancer patients before and 3 months after diagnosis showed cell-free circulating DNA released at apoptotic and non-apoptotic cell death. Interestingly, all patients after 6 months demonstrated DNA released at non-apoptotic cell. the principal source of cell-free circulating DNA is of apoptotic and non-apoptotic cell death. However, during treatment, this feature could change. Therefore, the study of cell-free circulating DNA would be important to follow the evolution of the disease during the treatment.
Keywords Prostate Cancer
Cell-free circulating DNA
DNA integrity
ALU sequence
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 10/50490-6
Date 2013-04-01
Published in Tumor Biology. Dordrecht: Springer, v. 34, n. 2, p. 983-986, 2013.
ISSN 1010-4283 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 983-986
Origin http://dx.doi.org/10.1007/s13277-012-0634-6
Access rights Closed access
Type Article
Web of Science ID WOS:000316364500044
URI http://repositorio.unifesp.br/handle/11600/36151

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