Patients with Chronic-form Paracoccidioidomycosis Present High Serum Levels of IgE Anti-paracoccidioides brasiliensis Gp70

Patients with Chronic-form Paracoccidioidomycosis Present High Serum Levels of IgE Anti-paracoccidioides brasiliensis Gp70

Autor Rigobello, F. F. Google Scholar
Marquez, A. S. Google Scholar
Lopes, J. D. Autor UNIFESP Google Scholar
Nakanishi-Ito, F. A. Google Scholar
Itano, E. N. Google Scholar
Instituição State Univ Londrina UEL
Univ Northern Parana UNOPAR
Universidade Federal de São Paulo (UNIFESP)
Resumo Paracoccidioidomycosis (PCM) is a disease caused by the Paracoccidioides genus, which includes P. brasiliensis and the new phylogenetic species P. lutzii. Resistance to this infection has been correlated with a Th1 pattern of cellular immune response, while susceptibility is correlated to an intense humoral immune response with an increase in IgE levels. Serum levels of IgE and IgG anti-gp70 and anti-exoantigen in chronic PCM were analyzed by enzyme-linked immunosorbent assay. Results showed a higher gp70 concentration in somatic antigen (SA) than in cell-free antigen (CFA) preparation and significantly higher levels of IgE and IgG anti-gp70 in chronic PCM patients' serum (n = 12) than in normal human serum (n = 12) (p < 0.05). Pearson's correlation analysis showed a strong correlation between IgG and IgE anti-gp70 (r = 0.8424). Additionally, IgE purified from a pool of acute and chronic PCM patient's serum was analyzed by immunoblotting. the patients with the acute form of the disease showed strong bands for gp43 and gp70 in SA but only for gp43 in CFA. in patients with the chronic form, solely the gp43 band was observed. in conclusion, we found that SA is a better source of gp70 than CFA is, and chronic PCM patients show high levels of IgE anti-gp70. This finding suggests that the Th2 immune response is potentially induced by gp70 in PCM disease, which calls for further study.
Palavra-chave Antibodies
gp70
Paracoccidioidomycosis
Somatic antigen
Virulence factor
Idioma Inglês
Financiador Fundacao Araucaria/PR
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Data de publicação 2013-04-01
Publicado em Mycopathologia. Dordrecht: Springer, v. 175, n. 3-4, p. 307-313, 2013.
ISSN 0301-486X (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 307-313
Fonte http://dx.doi.org/10.1007/s11046-013-9624-5
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000317623800014
Endereço permanente http://repositorio.unifesp.br/handle/11600/36148

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