High chromosome variability and the presence of multivalent associations in buthid scorpions

High chromosome variability and the presence of multivalent associations in buthid scorpions

Autor Mattos, Viviane Fagundes Google Scholar
Cella, Doralice Maria Google Scholar
Carvalho, Leonardo Sousa Google Scholar
Candido, Denise Maria Google Scholar
Schneider, Marielle Cristina Autor UNIFESP Google Scholar
Instituição Univ Estadual Paulista UNESP
Univ Fed Piaui UFPI
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Resumo In this study, we investigated the mitotic and meiotic chromosomes of 11 Buthidae scorpion species, belonging to three genera (Ananteris, Rhopalurus and Tityus), to obtain detailed knowledge regarding the mechanisms underlying the intraspecific and/or interspecific diversity of chromosome number and the origin of the complex chromosome associations observed during meiosis. the chromosomes of all species did not exhibit a localised centromere region and presented synaptic and achiasmatic behaviour during meiosis I. Spermatogonial and/or oogonial metaphase cells of these buthids showed diploid numbers range from 2n = 6 to 2n = 28. in most species, multivalent chromosome associations were observed in pachytene and postpachytene nuclei. Moreover, intraspecific variability associated with the presence or absence of chromosome chains and the number of chromosomes in the complex meiotic configurations was observed in some species of these three genera. Silver-impregnated cells revealed that the number and location of nucleolar organiser regions (NORs) remained unchanged despite extensive chromosome variation; notably, two NORs located on the terminal or subterminal chromosome regions were commonly observed for all species. C-banded and fluorochrome-stained cells showed that species with conspicuous blocks of heterochromatin exhibited the lowest rate of chromosomal rearrangement. Based on the investigation of mitotic and meiotic cells, we determined that the intraspecific variability occurred as a consequence of fission/fusion-type chromosomal rearrangements in Ananteris and Tityus species and reciprocal translocation in Rhopalurus species. Furthermore, we verified that individuals presenting the same diploid number differ in structural chromosome organisation, giving rise to intraspecific differences of chromosome association in meiotic cells (bivalent-like elements or chromosome chains).
Palavra-chave chromosome rearrangements
evolution
heterozygosity
holocentric
meiosis
nucleolar organiser region
Idioma Inglês
Financiador Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Programa de Pesquisas em Biodiversidade do Semiarido-PPBio Semiarido
Número do financiamento FAPESP: 2010/14226-2
FAPESP: 2011/21643-1
CNPq: 478630/2010-7
Programa de Pesquisas em Biodiversidade do Semiarido-PPBio Semiarido: CNPq 558317/2009-0
Data de publicação 2013-04-01
Publicado em Chromosome Research. Dordrecht: Springer, v. 21, n. 2, p. 121-136, 2013.
ISSN 0967-3849 (Sherpa/Romeo, fator de impacto)
Publicador Springer
Extensão 121-136
Fonte http://dx.doi.org/10.1007/s10577-013-9342-3
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000317688800003
Endereço permanente http://repositorio.unifesp.br/handle/11600/36147

Exibir registro completo




Arquivo

Arquivo Tamanho Formato Visualização

Não existem arquivos associados a este item.

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta